Pseudomonas aeruginosa PAO1, PA0779 (asrA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0779
Name
asrA
Replicon chromosome
Genomic location 845793 - 848192 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249470.1
GI 15595976
Affymetrix PA0779_at
Entrez 877837
GenBank AAG04168.1
INSDC AAG04168.1
NCBI Locus Tag PA0779
protein_id(GenBank) gb|AAG04168.1|AE004512_11|gnl|PseudoCAP|PA0779
TIGR NTL03PA00780
UniParc UPI00000C5149
UniProtKB Acc Q9I5F9
UniProtKB ID Q9I5F9_PSEAE
UniRef100 UniRef100_Q9I5F9
UniRef50 UniRef50_Q6ME13
UniRef90 UniRef90_Q9I5F9

Product

Feature Type CDS
Coding Frame 1
Product Name
AsrA
Product Name Confidence: Class 3
Synonyms aminoglycoside-induced stress response ATP-dependent protease
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.68
Kyte-Doolittle Hydrophobicity Value -0.239
Molecular Weight (kDa) 88.6
Isoelectric Point (pI) 6.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 541 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000753 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0779
Search term: asrA
Search term: AsrA

Human Homologs

Ensembl 97, assembly GRCh38.p12
lon peptidase 1, mitochondrial [Source:HGNC Symbol;Acc:HGNC:9479]
E-value: 2.8e-186
Percent Identity: 42.8

References

A human mitochondrial ATP-dependent protease that is highly homologous to bacterial Lon protease.
Wang N, Gottesman S, Willingham MC, Gottesman MM, Maurizi MR
Proc. Natl. Acad. Sci. U.S.A. 1993 Dec;90(23):11247-51
PubMed ID: 8248235
Cloning and sequence analysis of cDNA for a human homolog of eubacterial ATP-dependent Lon proteases.
Amerik AYu , Petukhova GV, Grigorenko VG, Lykov IP, Yarovoi SV, Lipkin VM, Gorbalenya AE
FEBS Lett. 1994 Feb;340(1-2):25-8
PubMed ID: 8119403
Involvement of an ATP-dependent protease, PA0779/AsrA, in inducing heat shock in response to tobramycin in Pseudomonas aeruginosa.
Kindrachuk KN, Fernández L, Bains M, Hancock RE
Antimicrob. Agents Chemother. 2011 May;55(5):1874-82
PubMed ID: 21357290