Pseudomonas aeruginosa PAO1, PA0782 (putA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0782
Name
putA
Synonym: pruA
Synonym: pruB
Replicon chromosome
Genomic location 851783 - 854965 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249473.1
GI 15595979
Affymetrix PA0782_putA_at
Entrez 878503
GenBank AAG04171.1
INSDC AAG04171.1
NCBI Locus Tag PA0782
protein_id(GenBank) gb|AAG04171.1|AE004513_3|gnl|PseudoCAP|PA0782
TIGR NTL03PA00783
UniParc UPI00000C514C
UniProtKB Acc Q9I5F6
UniProtKB ID Q9I5F6_PSEAE
UniRef100 UniRef100_Q9I5F6
UniRef50 UniRef50_Q9I5F6
UniRef90 UniRef90_Q9I5F6

Product

Feature Type CDS
Coding Frame 1
Product Name
proline dehydrogenase PutA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (115.63 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.17
Kyte-Doolittle Hydrophobicity Value -0.119
Molecular Weight (kDa) 115.6
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 594 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000756 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0782
Search term: putA

Human Homologs

Ensembl 94, assembly GRCh38.p12
aldehyde dehydrogenase 5 family member A1 [Source:HGNC Symbol;Acc:HGNC:408]
E-value: 5.1e-39
Percent Identity: 28.5

References

Divergent structure and regulatory mechanism of proline catabolic systems: characterization of the putAP proline catabolic operon of Pseudomonas aeruginosa PAO1 and its regulation by PruR, an AraC/XylS family protein.
Nakada Y, Nishijyo T, Itoh Y
J. Bacteriol. 2002 Oct;184(20):5633-40
PubMed ID: 12270821
Sequence analysis identifies the proline dehydrogenase and delta 1-pyrroline-5-carboxylate dehydrogenase domains of the multifunctional Escherichia coli PutA protein.
Ling M, Allen SW, Wood JM
J. Mol. Biol. 1994 Nov;243(5):950-6
PubMed ID: 7966312
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
PutA Is Required for Virulence and Regulated by PruR in Pseudomonas aeruginosa.
Zheng R, Feng X, Wei X, Pan X, Liu C, Song R, Jin Y, Bai F, Jin S, Wu W, Cheng Z.
Front Microbiol. 2018 Mar 26;9:548.
PubMed ID: 29632523