Pseudomonas aeruginosa PAO1, PA0783 (putP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0783
Name
putP
Replicon chromosome
Genomic location 855277 - 856797 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249474.1
GI 15595980
Affymetrix PA0783_putP_at
Entrez 877927
GenBank AAG04172.1
INSDC AAG04172.1
NCBI Locus Tag PA0783
protein_id(GenBank) gb|AAG04172.1|AE004513_4|gnl|PseudoCAP|PA0783
TIGR NTL03PA00784
UniParc UPI00000C514D
UniProtKB Acc Q9I5F5
UniProtKB ID Q9I5F5_PSEAE
UniRef100 UniRef100_Q9I5F5
UniRef50 UniRef50_P07117
UniRef90 UniRef90_Q9I5F5

Product

Feature Type CDS
Coding Frame 1
Product Name
sodium/proline symporter PutP
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.83
Kyte-Doolittle Hydrophobicity Value 0.749
Molecular Weight (kDa) 54.4
Isoelectric Point (pI) 7.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 486 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000757 (548 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0783
Search term: putP

Human Homologs

Ensembl 97, assembly GRCh38.p12
solute carrier family 5 member 3 [Source:HGNC Symbol;Acc:HGNC:11038]
E-value: 1.8e-13
Percent Identity: 24.1

References

Divergent structure and regulatory mechanism of proline catabolic systems: characterization of the putAP proline catabolic operon of Pseudomonas aeruginosa PAO1 and its regulation by PruR, an AraC/XylS family protein.
Nakada Y, Nishijyo T, Itoh Y
J Bacteriol 2002 Oct;184(20):5633-40
PubMed ID: 12270821
Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12.
Nakao T, Yamato I, Anraku Y
Mol Gen Genet 1987 Jun;208(1-2):70-5
PubMed ID: 3302614
Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli.
Yamato I, Kotani M, Oka Y, Anraku Y
J. Biol. Chem. 1994 Feb;269(8):5720-4
PubMed ID: 8119910
DNA sequence of proline permease gene from Pseudomonas fluorescens and predicted structure of proline permease.
Hosoya H, Nakamura K
Biosci. Biotechnol. Biochem. 1994 Nov;58(11):2099-101
PubMed ID: 7765603