Pseudomonas aeruginosa PAO1, PA0785 (azoR1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0785
Name
azoR1
Synonym: azoR
Replicon chromosome
Genomic location 857998 - 858636 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249476.1
GI 15595982
Affymetrix PA0785_at
DNASU PaCD00006357
Entrez 882036
GenBank AAG04174.1
INSDC AAG04174.1
NCBI Locus Tag PA0785
protein_id(GenBank) gb|AAG04174.1|AE004513_6|gnl|PseudoCAP|PA0785
TIGR NTL03PA00786
UniParc UPI00001251D2
UniProtKB Acc Q9I5F3
UniProtKB ID AZOR1_PSEAE
UniRef100 UniRef100_Q9I5F3
UniRef50 UniRef50_Q4KJ26
UniRef90 UniRef90_Q9I5F3

Product

Feature Type CDS
Coding Frame 1
Product Name
FMN-dependent NADH-azoreductase 1, AzoR1
Product Name Confidence: Class 1
Synonyms azobenzene reductase, AzoR
Evidence for Translation
Charge (pH 7) -2.83
Kyte-Doolittle Hydrophobicity Value -0.100
Molecular Weight (kDa) 23.0
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3KEG OXIDOREDUCTASE 10/26/09 X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 99.5
3LT5 OXIDOREDUCTASE 02/15/10 X-ray Crystallographic structure of a Pseudomonas Aeruginosa Azoreductase in complex with balsalazide PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
4N9Q OXIDOREDUCTASE 10/21/13 Crystal structure of paAzoR1 bound to ubiquinone-1 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
3R6W OXIDOREDUCTASE 03/22/11 paAzoR1 binding to nitrofurazone PSEUDOMONAS AERUGINOSA 2.085 X-RAY DIFFRACTION 100.0
2V9C OXIDOREDUCTASE 08/23/07 X-ray Crystallographic Structure of a Pseudomonas aeruginosa Azoreductase in Complex with Methyl Red. PSEUDOMONAS AERUGINOSA 2.18 X-RAY DIFFRACTION 100.0
4N65 OXIDOREDUCTASE 10/11/13 Crystal structure of paAzoR1 bound to anthraquinone-2-sulphonate PSEUDOMONAS AERUGINOSA 1.816 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
2-(4-DIMETHYLAMINOPHENYL)DIAZENYLBENZOIC ACID DB08209 DrugBank 5.1.4 Q9I5F3 FMN-dependent NADH-azoreductase 1 100.0 212 5.8e-114

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 245 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000759 (399 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0785
Search term: azoR1

References

Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli.
Fischl AS, Kennedy EP
J Bacteriol 1990 Sep;172(9):5445-9
PubMed ID: 2168383
Molecular cloning, characterisation and ligand-bound structure of an azoreductase from Pseudomonas aeruginosa.
Wang CJ, Hagemeier C, Rahman N, Lowe E, Noble M, Coughtrie M, Sim E, Westwood I
J. Mol. Biol. 2007 Nov;373(5):1213-28
PubMed ID: 17904577
A novel mechanism for azoreduction.
Ryan A, Laurieri N, Westwood I, Wang CJ, Lowe E, Sim E
J. Mol. Biol. 2010 Jul;400(1):24-37
PubMed ID: 20417637