Pseudomonas aeruginosa PAO1, PA0807 (ampDh3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0807
Name
ampDh3
Replicon chromosome
Genomic location 884799 - 885566 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249498.1
GI 15596004
Affymetrix PA0807_at
Entrez 877713
GenBank AAG04196.1
INSDC AAG04196.1
NCBI Locus Tag PA0807
protein_id(GenBank) gb|AAG04196.1|AE004516_1|gnl|PseudoCAP|PA0807
TIGR NTL03PA00808
UniParc UPI00000C5164
UniProtKB Acc Q9I5D1
UniProtKB ID Q9I5D1_PSEAE
UniRef100 UniRef100_Q9I5D1
UniRef50 UniRef50_Q9I5D1
UniRef90 UniRef90_Q9I5D1

Product

Feature Type CDS
Coding Frame 1
Product Name
AmpDh3
Product Name Confidence: Class 1
Synonyms AmpDh3 (changed from conserved hypothetical protein)
Evidence for Translation
Detected using LC-MS/MS (28.72 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.57
Kyte-Doolittle Hydrophobicity Value -0.415
Molecular Weight (kDa) 28.7
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4BXJ HYDROLASE 07/12/13 CRYSTAL STRUCTURE OF AMPDH3 FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA PAO1 2.35 X-RAY DIFFRACTION 100.0
4BXE HYDROLASE/PEPTIDE 07/10/13 CRYSTAL STRUCTURE OF AMPDH3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH ANHYDROMURAMIC PENTAPEPTIDE PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT 2.95 X-RAY DIFFRACTION 100.0
4BXD HYDROLASE/PEPTIDE 07/10/13 CRYSTAL STRUCTURE OF AMPDH3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH TETRASACCHARIDE PENTAPEPTIDE PSEUDOMONAS AERUGINOSA PAO1; SYNTHETIC CONSTRUCT 3.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 226 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000780 (368 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0807
Search term: ampDh3
Search term: AmpDh3

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Stepwise upregulation of the Pseudomonas aeruginosa chromosomal cephalosporinase conferring high-level beta-lactam resistance involves three AmpD homologues.
Juan C, Moyá B, Pérez JL, Oliver A
Antimicrob Agents Chemother 2006 May;50(5):1780-7
PubMed ID: 16641450
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344