Pseudomonas aeruginosa PAO1, PA0844 (plcH)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: plcS
Synonym: phlC
Replicon chromosome
Genomic location 919258 - 921450 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPlcH also exerts sphingomyelin synthase activity.


RefSeq NP_249535.1
GI 15596041
Affymetrix PA0844_plcH_at
Entrez 879296
GenBank AAG04233.1
INSDC AAG04233.1
NCBI Locus Tag PA0844
protein_id(GenBank) gb|AAG04233.1|AE004519_4|gnl|PseudoCAP|PA0844
UniParc UPI000013197F
UniProtKB Acc P06200
UniRef100 UniRef100_P06200
UniRef50 UniRef50_P06200
UniRef90 UniRef90_P06200


Feature Type CDS
Coding Frame 1
Product Name
hemolytic phospholipase C precursor
Product Name Confidence: Class 1
Synonyms sphingomyelin synthase
Evidence for Translation
Charge (pH 7) -4.35
Kyte-Doolittle Hydrophobicity Value -0.548
Molecular Weight (kDa) 82.7
Isoelectric Point (pI) 6.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Extracellular Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hemolytic phospholipase C
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Infection model Mouse model of acute systemic infection
Reference 21041488
Source 2
Database VFDB : VF0092
Category Hemolytic phospholipase C
Evidence ECO:0001175
deletion evidence used in manual assertion
Host Organism Homo sapiens
Notes Targeted deletion of the plcHR operon induced a much more robust oxidative burst from human neutrophils.
Reference 10225897

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000811 (301 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0844
Search term: plcH


Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme.
Luberto C, Stonehouse MJ, Collins EA, Marchesini N, El-Bawab S, Vasil AI, Vasil ML, Hannun YA
J Biol Chem 2003 Aug 29;278(35):32733-43
PubMed ID: 12799377
Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa.
Pritchard AE, Vasil ML
J Bacteriol 1986 Jul;167(1):291-8
PubMed ID: 3087958
Nucleotide sequences and expression in Escherichia coli of the in-phase overlapping Pseudomonas aeruginosa plcR genes.
Shen BF, Tai PC, Pritchard AE, Vasil ML
J Bacteriol 1987 Oct;169(10):4602-7
PubMed ID: 3115961
Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions.
Ostroff RM, Wretlind B, Vasil ML
Infect Immun 1989 May;57(5):1369-73
PubMed ID: 2496027
PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa.
Cota-Gomez A, Vasil AI, Kadurugamuwa J, Beveridge TJ, Schweizer HP, Vasil ML
Infect. Immun. 1997 Jul;65(7):2904-13
PubMed ID: 9199466
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway.
Voulhoux R, Ball G, Ize B, Vasil ML, Lazdunski A, Wu LF, Filloux A
EMBO J. 2001 Dec;20(23):6735-41
PubMed ID: 11726509