Pseudomonas aeruginosa PAO1, PA0844 (plcH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0844
Name
plcH
Synonym: plcS
Synonym: phlC
Replicon chromosome
Genomic location 919258 - 921450 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPlcH also exerts sphingomyelin synthase activity.

Cross-References

RefSeq NP_249535.1
GI 15596041
Affymetrix PA0844_plcH_at
Entrez 879296
GenBank AAG04233.1
INSDC AAG04233.1
NCBI Locus Tag PA0844
protein_id(GenBank) gb|AAG04233.1|AE004519_4|gnl|PseudoCAP|PA0844
TIGR NTL03PA00845
UniParc UPI000013197F
UniProtKB Acc P06200
UniProtKB ID PHLC_PSEAE
UniRef100 UniRef100_P06200
UniRef50 UniRef50_P06200
UniRef90 UniRef90_P06200

Product

Feature Type CDS
Coding Frame 1
Product Name
hemolytic phospholipase C precursor
Product Name Confidence: Class 1
Synonyms sphingomyelin synthase
Evidence for Translation
Charge (pH 7) -4.35
Kyte-Doolittle Hydrophobicity Value -0.548
Molecular Weight (kDa) 82.7
Isoelectric Point (pI) 6.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hemolytic phospholipase C
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of acute systemic infection
Reference 21041488
Source 2
Database VFDB : VF0092
Category Hemolytic phospholipase C
Evidence ECO:0001175
deletion evidence used in manual assertion
Host Organism Homo sapiens
Notes Targeted deletion of the plcHR operon induced a much more robust oxidative burst from human neutrophils.
Reference 10225897

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000811 (301 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0844
Search term: plcH

References

Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme.
Luberto C, Stonehouse MJ, Collins EA, Marchesini N, El-Bawab S, Vasil AI, Vasil ML, Hannun YA
J Biol Chem 2003 Aug 29;278(35):32733-43
PubMed ID: 12799377
Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa.
Pritchard AE, Vasil ML
J Bacteriol 1986 Jul;167(1):291-8
PubMed ID: 3087958
Nucleotide sequences and expression in Escherichia coli of the in-phase overlapping Pseudomonas aeruginosa plcR genes.
Shen BF, Tai PC, Pritchard AE, Vasil ML
J Bacteriol 1987 Oct;169(10):4602-7
PubMed ID: 3115961
Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions.
Ostroff RM, Wretlind B, Vasil ML
Infect Immun 1989 May;57(5):1369-73
PubMed ID: 2496027
PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa.
Cota-Gomez A, Vasil AI, Kadurugamuwa J, Beveridge TJ, Schweizer HP, Vasil ML
Infect. Immun. 1997 Jul;65(7):2904-13
PubMed ID: 9199466
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway.
Voulhoux R, Ball G, Ize B, Vasil ML, Lazdunski A, Wu LF, Filloux A
EMBO J. 2001 Dec;20(23):6735-41
PubMed ID: 11726509