Pseudomonas aeruginosa PAO1, PA0844 (plcH)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0844
Name	plcH Synonym: plcS Synonym: phlC
Replicon	chromosome
Genomic location	919258 - 921450 (- strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	PlcH also exerts sphingomyelin synthase activity.

Cross-References

RefSeq	NP_249535.1
GI	15596041
Affymetrix	PA0844_plcH_at
Entrez	879296
GenBank	AAG04233.1
INSDC	AAG04233.1
NCBI Locus Tag	PA0844
protein_id(GenBank)	gb|AAG04233.1|AE004519_4|gnl|PseudoCAP|PA0844
TIGR	NTL03PA00845
UniParc	UPI000013197F
UniProtKB Acc	P06200
UniProtKB ID	PHLC_PSEAE
UniRef100	UniRef100_P06200
UniRef50	UniRef50_P06200
UniRef90	UniRef90_P06200

Product

Feature Type	CDS
Coding Frame	1
Product Name	hemolytic phospholipase C precursor Product Name Confidence: Class 1
Synonyms	sphingomyelin synthase
Evidence for Translation
Charge (pH 7)	-4.35
Kyte-Doolittle Hydrophobicity Value	-0.548
Molecular Weight (kDa)	82.7
Isoelectric Point (pI)	6.60

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 1 Extracellular Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	PseudoCAP
Category	Hemolytic phospholipase C
Evidence	ECO:0000315 mutant phenotype evidence used in manual assertion
Host Organism	Mus musculus
Notes
Infection model	Mouse model of acute systemic infection
Reference	21041488
Source 2
Database	VFDB : VF0092
Category	Hemolytic phospholipase C
Evidence	ECO:0001175 deletion evidence used in manual assertion
Host Organism	Homo sapiens
Notes	Targeted deletion of the plcHR operon induced a much more robust oxidative burst from human neutrophils.
Reference	10225897

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 96 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000811 (298 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0844 Search term: plcH Search term: hemolytic phospholipase C precursor

Human Homologs

References

Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme. Luberto C, Stonehouse MJ, Collins EA, Marchesini N, El-Bawab S, Vasil AI, Vasil ML, Hannun YA J Biol Chem 2003 Aug 29;278(35):32733-43 PubMed ID: 12799377

Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa. Pritchard AE, Vasil ML J Bacteriol 1986 Jul;167(1):291-8 PubMed ID: 3087958

Nucleotide sequences and expression in Escherichia coli of the in-phase overlapping Pseudomonas aeruginosa plcR genes. Shen BF, Tai PC, Pritchard AE, Vasil ML J Bacteriol 1987 Oct;169(10):4602-7 PubMed ID: 3115961

Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions. Ostroff RM, Wretlind B, Vasil ML Infect Immun 1989 May;57(5):1369-73 PubMed ID: 2496027

PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa. Cota-Gomez A, Vasil AI, Kadurugamuwa J, Beveridge TJ, Schweizer HP, Vasil ML Infect Immun 1997 Jul;65(7):2904-13 PubMed ID: 9199466

Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. Voulhoux R, Ball G, Ize B, Vasil ML, Lazdunski A, Wu LF, Filloux A EMBO J 2001 Dec 3;20(23):6735-41 PubMed ID: 11726509