Pseudomonas aeruginosa PAO1, PA0888 (aotJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0888
Name
aotJ
Replicon chromosome
Genomic location 972166 - 972945 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249579.1
GI 15596085
Affymetrix PA0888_aotJ_at
DNASU PaCD00006408
Entrez 879210
GenBank AAG04277.1
INSDC AAG04277.1
NCBI Locus Tag PA0888
protein_id(GenBank) gb|AAG04277.1|AE004523_5|gnl|PseudoCAP|PA0888
TIGR NTL03PA00889
UniParc UPI00000D4712
UniProtKB Acc G3XD47
UniProtKB ID G3XD47_PSEAE
UniRef100 UniRef100_W0YLE0
UniRef50 UniRef50_A6VA85
UniRef90 UniRef90_A6VA85

Product

Feature Type CDS
Coding Frame 1
Product Name
arginine/ornithine binding protein AotJ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (28.01 kDa, PMID:21751344)
Charge (pH 7) -0.06
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 28.0
Isoelectric Point (pI) 6.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Outer Membrane Vesicle Class 1
Periplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 452 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000855 (520 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0888
Search term: aotJ

References

Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
Sriramulu DD, Nimtz M, Romling U
Proteomics 2005 Sep;5(14):3712-21
PubMed ID: 16097035
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J. Bacteriol. 2015 Feb;197(4):762-73
PubMed ID: 25488299