Pseudomonas aeruginosa PAO1, PA0896 (aruF)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0896
Name	aruF Synonym: aruAI
Replicon	chromosome
Genomic location	979329 - 980345 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_249587.1
GI	15596093
Affymetrix	PA0896_aruF_at
DNASU	PaCD00006413
Entrez	879437
GenBank	AAG04285.1
INSDC	AAG04285.1
NCBI Locus Tag	PA0896
protein_id(GenBank)	gb|AAG04285.1|AE004524_2|gnl|PseudoCAP|PA0896
TIGR	NTL03PA00897
UniParc	UPI00001261AE
UniProtKB Acc	P80357
UniProtKB ID	ASTA_PSEAE
UniRef100	UniRef100_P80357
UniRef50	UniRef50_P80357
UniRef90	UniRef90_P80357

Product

Feature Type	CDS
Coding Frame	1
Product Name	subunit I of arginine N2-succinyltransferase = ornithine N2-succinyltransferase Product Name Confidence: Class 1
Synonyms	arginine/ornithine succinyltransferase AI subunit
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-14.13
Kyte-Doolittle Hydrophobicity Value	0.003
Molecular Weight (kDa)	36.9
Isoelectric Point (pI)	4.66

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1YLE	TRANSFERASE	01/19/05	The structure of arginine/ornithine succinyltransferase subunit AI from Pseudomonas aeruginosa.	Pseudomonas aeruginosa	1.7	X-RAY DIFFRACTION	100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Formic acid	DB01942	DrugBank	5.1.4	P80357	Arginine N-succinyltransferase subunit alpha	100.0	338	1.2e-188

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000863 (512 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0896 Search term: aruF Search term: subunit I of arginine N2-succinyltransferase = ornithine N2-succinyltransferase

Human Homologs

References

Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1. Park SM, Lu CD, Abdelal AT J Bacteriol 1997 Sep;179(17):5300-8 PubMed ID: 9286980

Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa. Itoh Y J Bacteriol 1997 Dec;179(23):7280-90 PubMed ID: 9393691

N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa. Jann A, Stalon V, Wauven CV, Leisinger T, Haas D Proc Natl Acad Sci U S A 1986 Jul;83(13):4937-41 PubMed ID: 16593724