Pseudomonas aeruginosa PAO1, PA0905 (rsmA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0905
Name
rsmA
Synonym: csrA
Replicon chromosome
Genomic location 991013 - 991198 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPost-transcriptional riboregulator

Cross-References

RefSeq NP_249596.1
GI 15596102
Affymetrix PA0905_csrA_at
DNASU PaCD00006416
Entrez 878352
GenBank AAG04294.1
INSDC AAG04294.1
NCBI Locus Tag PA0905
protein_id(GenBank) gb|AAG04294.1|AE004525_2|gnl|PseudoCAP|PA0905
TIGR NTL03PA00906
UniParc UPI00001285B6
UniProtKB Acc O69078
UniProtKB ID CSRA_PSEAE
UniRef100 UniRef100_B7UY29
UniRef50 UniRef50_Q5E7G3
UniRef90 UniRef90_A4VJB5

Product

Feature Type CDS
Coding Frame 1
Product Name
RsmA
Product Name Confidence: Class 1
Synonyms carbon storage regulator
Evidence for Translation
Charge (pH 7) 0.46
Kyte-Doolittle Hydrophobicity Value -0.446
Molecular Weight (kDa) 6.9
Isoelectric Point (pI) 7.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1VPZ RNA BINDING PROTEIN 11/24/04 Crystal structure of a putative carbon storage regulator protein (csra, pa0905) from pseudomonas aeruginosa at 2.05 A resolution PSEUDOMONAS AERUGINOSA 2.05 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category RsmA
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse model of chronic infection
Infection model Mouse model of acute pneumonia
Reference 18025099

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 227 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000872 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0905
Search term: rsmA
Search term: RsmA

References

Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1.
Heurlier K, Williams F, Heeb S, Dormond C, Pessi G, Singer D, Cámara M, Williams P, Haas D
J. Bacteriol. 2004 May;186(10):2936-45
PubMed ID: 15126453
The global posttranscriptional regulator RsmA modulates production of virulence determinants and N-acylhomoserine lactones in Pseudomonas aeruginosa.
Pessi G, Williams F, Hindle Z, Heurlier K, Holden MT, Cámara M, Haas D, Williams P
J. Bacteriol. 2001 Nov;183(22):6676-83
PubMed ID: 11673439
Identification and molecular characterization of csrA, a pleiotropic gene from Escherichia coli that affects glycogen biosynthesis, gluconeogenesis, cell size, and surface properties.
Romeo T, Gong M, Liu MY, Brun-Zinkernagel AM
J. Bacteriol. 1993 Aug;175(15):4744-55
PubMed ID: 8393005
The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli.
Liu MY, Gui G, Wei B, Preston JF, Oakford L, Yüksel U, Giedroc DP, Romeo T
J. Biol. Chem. 1997 Jul;272(28):17502-10
PubMed ID: 9211896
Low oxygen induces the type III secretion system in Pseudomonas aeruginosa via modulation of the small RNAs rsmZ and rsmY.
O'Callaghan J, Reen FJ, Adams C, O'Gara F
Microbiology (Reading, Engl.) 2011 Dec;157(Pt 12):3417-28
PubMed ID: 21873408
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999