Pseudomonas aeruginosa PAO1, PA0913 (mgtE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0913
Name
mgtE
Replicon chromosome
Genomic location 996038 - 997486 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249604.1
GI 15596110
Affymetrix PA0913_mgtE_at
Entrez 878306
GenBank AAG04302.1
INSDC AAG04302.1
NCBI Locus Tag PA0913
protein_id(GenBank) gb|AAG04302.1|AE004525_10|gnl|PseudoCAP|PA0913
TIGR NTL03PA00914
UniParc UPI00000C51B4
UniProtKB Acc Q9I544
UniProtKB ID Q9I544_PSEAE
UniRef100 UniRef100_W0YKI5
UniRef50 UniRef50_I7KFQ9
UniRef90 UniRef90_W0YKI5

Product

Feature Type CDS
Coding Frame 1
Product Name
MgtE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (53.82 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.83
Kyte-Doolittle Hydrophobicity Value 0.036
Molecular Weight (kDa) 53.8
Isoelectric Point (pI) 4.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 484 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000879 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0913
Search term: mgtE
Search term: MgtE

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning of the mgtE Mg2+ transporter from Providencia stuartii and the distribution of mgtE in gram-negative and gram-positive bacteria.
Townsend DE, Esenwine AJ, George J, Bross D, Maguire ME, Smith RL
J. Bacteriol. 1995 Sep;177(18):5350-4
PubMed ID: 7665526
Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.
Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S
DNA Res. 1996 Jun;3(3):109-36
PubMed ID: 8905231
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
MgtE is a dual-function protein in Pseudomonas aeruginosa.
Coffey BM, Akhand SS, Anderson GG
Microbiology (Reading, Engl.) 2014 Jun;160(Pt 6):1200-13
PubMed ID: 24722909
The Pseudomonas aeruginosa magnesium transporter MgtE inhibits transcription of the type III secretion system.
Anderson GG, Yahr TL, Lovewell RR, O'Toole GA
Infect. Immun. 2010 Mar;78(3):1239-49
PubMed ID: 20028803