Pseudomonas aeruginosa PAO1, PA0917 (kup)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0917
Name
kup
Replicon chromosome
Genomic location 1000013 - 1001917 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249608.1
GI 15596114
Affymetrix PA0917_kup_at
Entrez 880673
GenBank AAG04306.1
INSDC AAG04306.1
NCBI Locus Tag PA0917
protein_id(GenBank) gb|AAG04306.1|AE004526_1|gnl|PseudoCAP|PA0917
TIGR NTL03PA00918
UniParc UPI00000C51B8
UniProtKB Acc Q9I540
UniProtKB ID KUP_PSEAE
UniRef100 UniRef100_B7UY17
UniRef50 UniRef50_Q87Y19
UniRef90 UniRef90_B7UY17

Product

Feature Type CDS
Coding Frame 1
Product Name
potassium uptake protein Kup
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.52
Kyte-Doolittle Hydrophobicity Value 0.629
Molecular Weight (kDa) 68.7
Isoelectric Point (pI) 7.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 205 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000883 (519 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0917
Search term: kup

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus.
Schleyer M, Bakker EP
J. Bacteriol. 1993 Nov;175(21):6925-31
PubMed ID: 8226635
Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.
Blattner FR, Burland V, Plunkett G, Sofia HJ, Daniels DL
Nucleic Acids Res. 1993 Nov;21(23):5408-17
PubMed ID: 8265357
AtKuP1: a dual-affinity K+ transporter from Arabidopsis.
Fu HH, Luan S
Plant Cell 1998 Jan;10(1):63-73
PubMed ID: 9477572