Pseudomonas aeruginosa PAO1, PA0917 (kup)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0917
Name
kup
Replicon chromosome
Genomic location 1000013 - 1001917 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249608.1
GI 15596114
Affymetrix PA0917_kup_at
Entrez 880673
GenBank AAG04306.1
INSDC AAG04306.1
NCBI Locus Tag PA0917
protein_id(GenBank) gb|AAG04306.1|AE004526_1|gnl|PseudoCAP|PA0917
TIGR NTL03PA00918
UniParc UPI00000C51B8
UniProtKB Acc Q9I540
UniProtKB ID KUP_PSEAE
UniRef100 UniRef100_B7UY17
UniRef50 UniRef50_Q87Y19
UniRef90 UniRef90_B7UY17

Product

Feature Type CDS
Coding Frame 1
Product Name
potassium uptake protein Kup
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.52
Kyte-Doolittle Hydrophobicity Value 0.629
Molecular Weight (kDa) 68.7
Isoelectric Point (pI) 7.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 205 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000883 (504 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0917
Search term: kup

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus.
Schleyer M, Bakker EP
J Bacteriol 1993 Nov;175(21):6925-31
PubMed ID: 8226635
Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.
Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL
Nucleic Acids Res 1993 Nov 25;21(23):5408-17
PubMed ID: 8265357
AtKuP1: a dual-affinity K+ transporter from Arabidopsis.
Fu HH, Luan S
Plant Cell 1998 Jan;10(1):63-73
PubMed ID: 9477572