Pseudomonas aeruginosa PAO1, PA0927 (ldhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0927
Name
ldhA
Synonym: ldhD
Replicon chromosome
Genomic location 1011983 - 1012972 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249618.1
GI 15596124
Affymetrix PA0927_ldhA_at
Entrez 881895
GenBank AAG04316.1
INSDC AAG04316.1
NCBI Locus Tag PA0927
protein_id(GenBank) gb|AAG04316.1|AE004527_3|gnl|PseudoCAP|PA0927
TIGR NTL03PA00928
UniParc UPI00000C51C1
UniProtKB Acc Q9I530
UniProtKB ID Q9I530_PSEAE
UniRef100 UniRef100_Q9I530
UniRef50 UniRef50_Q1IDM4
UniRef90 UniRef90_Q9I530

Product

Feature Type CDS
Coding Frame 1
Product Name
D-lactate dehydrogenase (fermentative)
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.72
Kyte-Doolittle Hydrophobicity Value 0.042
Molecular Weight (kDa) 35.8
Isoelectric Point (pI) 6.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6ABJ OXIDOREDUCTASE 07/21/18 The apo-structure of D-lactate dehydrogenase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.86 X-RAY DIFFRACTION 100.0
3WWZ OXIDOREDUCTASE 07/08/14 The crystal structure of D-lactate dehydrogenase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 2.0 X-RAY DIFFRACTION 100.0
5Z20 OXIDOREDUCTASE 12/28/17 The ternary structure of D-lactate dehydrogenase from Pseudomonas aeruginosa with NADH and oxamate PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 640 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000893 (1040 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0927
Search term: ldhA

References

Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase.
Mat-Jan F, Alam KY, Clark DP
J Bacteriol 1989 Jan;171(1):342-8
PubMed ID: 2644194
Characterization of twenty-six new heat shock genes of Escherichia coli.
Chuang SE, Blattner FR
J. Bacteriol. 1993 Aug;175(16):5242-52
PubMed ID: 8349564
The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli.
Bunch PK, Mat-Jan F, Lee N, Clark DP
Microbiology (Reading, Engl.) 1997 Jan;143 ( Pt 1)
PubMed ID: 9025293