Pseudomonas aeruginosa PAO1, PA0985 (pyoS5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0985
Name
pyoS5
Replicon chromosome
Genomic location 1066321 - 1067817 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249676.1
GI 15596182
Affymetrix PA0985_at
Entrez 877569
GenBank AAG04374.1
INSDC AAG04374.1
NCBI Locus Tag PA0985
protein_id(GenBank) gb|AAG04374.1|AE004531_11|gnl|PseudoCAP|PA0985
TIGR NTL03PA00986
UniParc UPI00000C51ED
UniProtKB Acc Q9I4Y4
UniProtKB ID Q9I4Y4_PSEAE
UniRef100 UniRef100_Q9I4Y4
UniRef50 UniRef50_Q9I4Y4
UniRef90 UniRef90_Q9I4Y4

Product

Feature Type CDS
Coding Frame 1
Product Name
pyocin S5
Product Name Confidence: Class 2
Synonyms probable colicin-like toxin
Evidence for Translation
Detected using LC-MS/MS (56.07 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.52
Kyte-Doolittle Hydrophobicity Value -0.514
Molecular Weight (kDa) 56.1
Isoelectric Point (pI) 9.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 12 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000948 (432 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0985
Search term: pyoS5
Search term: pyocin S5

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage.
Ling H, Saeidi N, Rasouliha BH, Chang MW
FEBS Lett. 2010 Aug;584(15):3354-8
PubMed ID: 20580355
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344