Pseudomonas aeruginosa PAO1, PA0996 (pqsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0996
Name
pqsA
Replicon chromosome
Genomic location 1078462 - 1080015 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPseudomonas quinolone signal response

Cross-References

RefSeq NP_249687.1
GI 15596193
Affymetrix PA0996_at
Entrez 880760
GenBank AAG04385.1
INSDC AAG04385.1
NCBI Locus Tag PA0996
protein_id(GenBank) gb|AAG04385.1|AE004532_9|gnl|PseudoCAP|PA0996
TIGR NTL03PA00997
UniParc UPI00000C51F8
UniProtKB Acc Q9I4X3
UniProtKB ID PQSA_PSEAE
UniRef100 UniRef100_Q9I4X3
UniRef50 UniRef50_Q9I4X3
UniRef90 UniRef90_Q9I4X3

Product

Feature Type CDS
Coding Frame 1
Product Name
PqsA
Product Name Confidence: Class 1
Synonyms probable coenzyme A ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.98
Kyte-Doolittle Hydrophobicity Value -0.068
Molecular Weight (kDa) 56.6
Isoelectric Point (pI) 6.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5OE5 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 3) PSEUDOMONAS AERUGINOSA PAO1 1.74 X-RAY DIFFRACTION 100.0
5OE3 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 1) PSEUDOMONAS AERUGINOSA PAO1 1.43 X-RAY DIFFRACTION 100.0
5OE4 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with anthraniloyl-AMP (crystal form 2) PSEUDOMONAS AERUGINOSA PAO1 1.904 X-RAY DIFFRACTION 100.0
5OE6 LIGASE 07/07/17 Crystal structure of the N-terminal domain of PqsA in complex with 6-fluoroanthraniloyl-AMP (crystal form 1) PSEUDOMONAS AERUGINOSA PAO1 1.67 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Populus trichocarpa
Notes
Infection model Poplar pathogenicity wilting assay
Reference 21261818

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 600 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000959 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0996
Search term: pqsA
Search term: PqsA

Human Homologs

Ensembl 94, assembly GRCh38.p12
acyl-CoA synthetase short chain family member 1 [Source:HGNC Symbol;Acc:HGNC:16091]
E-value: 1.3e-24
Percent Identity: 24.3

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
4-Hydroxybenzoate-coenzyme A ligase from Rhodopseudomonas palustris: purification, gene sequence, and role in anaerobic degradation.
Gibson J, Dispensa M, Fogg GC, Evans DT, Harwood CS
J. Bacteriol. 1994 Feb;176(3):634-41
PubMed ID: 8300518
PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a newly identified metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis.
Lépine F, Dekimpe V, Lesic B, Milot S, Lesimple A, Mamer OA, Rahme LG, Déziel E
Biol. Chem. 2007 Aug;388(8):839-45
PubMed ID: 17655503