Pseudomonas aeruginosa PAO1, PA0999 (pqsD)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: fabH1
Replicon chromosome
Genomic location 1081942 - 1082955 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentQuinolone signal response
Commentboth pqsC and pqsD are needed for quinolone signal biosynthesis


RefSeq NP_249690.1
GI 15596196
Affymetrix PA0999_fabH1_at
DNASU PaCD00006462
Entrez 880625
GenBank AAG04388.1
INSDC AAA88446.1
NCBI Locus Tag PA0999
protein_id(GenBank) gb|AAG04388.1|AE004532_12|gnl|PseudoCAP|PA0999
UniParc UPI000012A472
UniProtKB Acc P20582
UniRef100 UniRef100_P20582
UniRef50 UniRef50_P20582
UniRef90 UniRef90_P20582


Feature Type CDS
Coding Frame 1
Product Name
3-oxoacyl-[acyl-carrier-protein] synthase III
Product Name Confidence: Class 1
Synonyms beta-ketoacyl-ACP synthase III
acetyl CoA ACP transacetylase
Evidence for Translation
Charge (pH 7) -9.53
Kyte-Doolittle Hydrophobicity Value 0.072
Molecular Weight (kDa) 36.4
Isoelectric Point (pI) 5.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3H77 TRANSFERASE 04/24/09 Crystal structure of Pseudomonas aeruginosa PqsD in a covalent complex with anthranilate PSEUDOMONAS AERUGINOSA PAO1 1.8 X-RAY DIFFRACTION 100.0
3H76 TRANSFERASE 04/24/09 Crystal structure of PqsD, a key enzyme in Pseudomonas aeruginosa quinolone signal biosynthesis pathway PSEUDOMONAS AERUGINOSA PAO1 1.8 X-RAY DIFFRACTION 100.0
3H78 TRANSFERASE 04/24/09 Crystal structure of Pseudomonas aeruginosa PqsD C112A mutant in complex with anthranilic acid PSEUDOMONAS AERUGINOSA PAO1 1.7 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 587 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000962 (611 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0999
Search term: pqsD


Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications.
Essar DW, Eberly L, Hadero A, Crawford IP
J. Bacteriol. 1990 Feb;172(2):884-900
PubMed ID: 2153661
Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12.
Tsay JT, Oh W, Larson TJ, Jackowski S, Rock CO
J. Biol. Chem. 1992 Apr;267(10):6807-14
PubMed ID: 1551888