Pseudomonas aeruginosa PAO1, PA1032 (quiP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1032
Name
quiP
Synonym: pac
Replicon chromosome
Genomic location 1119674 - 1122217 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentquiP encodes a protein with acyl-HSL acylase activity for long but not short acyl-HSLs.
CommentEncodes an enzyme with a homoserine lactone-releasing, acyl-homoserine lactone(AHL)acylase activity specific towards long-chain AHLs

Cross-References

RefSeq NP_249723.1
GI 15596229
Affymetrix PA1032_at
Entrez 880272
GenBank AAG04421.1
INSDC AAG04421.1
NCBI Locus Tag PA1032
protein_id(GenBank) gb|AAG04421.1|AE004535_8|gnl|PseudoCAP|PA1032
TIGR NTL03PA01033
UniParc UPI00000C5213
UniProtKB Acc Q9I4U2
UniProtKB ID QUIP_PSEAE
UniRef100 UniRef100_Q9I4U2
UniRef50 UniRef50_Q9I4U2
UniRef90 UniRef90_Q9I4U2

Product

Feature Type CDS
Coding Frame 1
Product Name
QuiP
Product Name Confidence: Class 1
Synonyms acyl-homoserine lactone acylase, QuiP
QuiP (changed from probable penicillin amidase)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 3.31
Kyte-Doolittle Hydrophobicity Value -0.410
Molecular Weight (kDa) 94.1
Isoelectric Point (pI) 7.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000994 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1032
Search term: quiP
Search term: QuiP

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosa PAO1.
Huang JJ, Petersen A, Whiteley M, Leadbetter JR
Appl Environ Microbiol 2006 Feb;72(2):1190-7
PubMed ID: 16461666
Penicillin acylase from E. coli: unique gene-protein relation.
Schumacher G, Sizmann D, Haug H, Buckel P, Böck A
Nucleic Acids Res 1986 Jul 25;14(14):5713-27
PubMed ID: 3016663
Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
Matsuda A, Matsuyama K, Yamamoto K, Ichikawa S, Komatsu K
J Bacteriol 1987 Dec;169(12):5815-20
PubMed ID: 2824449
Nucleotide sequences of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
Matsuda A, Toma K, Komatsu K
J Bacteriol 1987 Dec;169(12):5821-6
PubMed ID: 3680178
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994