Pseudomonas aeruginosa PAO1, PA1059 (shaF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1059
Name
shaF
Synonym: phaG
Replicon chromosome
Genomic location 1147468 - 1147815 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249750.1
GI 15596256
Affymetrix PA1059_at
DNASU PaCD00006485
Entrez 881016
GenBank AAG04448.1
INSDC AAG04448.1
NCBI Locus Tag PA1059
protein_id(GenBank) gb|AAG04448.1|AE004537_13|gnl|PseudoCAP|PA1059
TIGR NTL03PA01060
UniParc UPI00000C522D
UniProtKB Acc Q9I4R5
UniProtKB ID Q9I4R5_PSEAE
UniRef100 UniRef100_W0YLW9
UniRef50 UniRef50_Q02IM6
UniRef90 UniRef90_Q02IM6

Product

Feature Type CDS
Coding Frame 1
Product Name
ShaF
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.46
Kyte-Doolittle Hydrophobicity Value 0.717
Molecular Weight (kDa) 12.5
Isoelectric Point (pI) 10.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 220 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001021 (425 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1059
Search term: shaF
Search term: ShaF

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003.
Vlcek C, Paces V, Maltsev N, Paces J, Haselkorn R, Fonstein M
Proc. Natl. Acad. Sci. U.S.A. 1997 Aug;94(17):9384-8
PubMed ID: 9256491
Characterization of a multigene-encoded sodium/hydrogen antiporter (sha) from Pseudomonas aeruginosa: its involvement in pathogenesis.
Kosono S, Haga K, Tomizawa R, Kajiyama Y, Hatano K, Takeda S, Wakai Y, Hino M, Kudo T
J. Bacteriol. 2005 Aug;187(15):5242-8
PubMed ID: 16030218