Pseudomonas aeruginosa PAO1, PA1071 (braF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1071
Name
braF
Replicon chromosome
Genomic location 1158658 - 1159425 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249762.1
GI 15596268
Affymetrix PA1071_braF_at
Entrez 878078
GenBank AAG04460.1
INSDC AAG04460.1
NCBI Locus Tag PA1071
protein_id(GenBank) gb|AAG04460.1|AE004539_2|gnl|PseudoCAP|PA1071
TIGR NTL03PA01072
UniParc UPI0000126ABA
UniProtKB Acc P21629
UniProtKB ID BRAF_PSEAE
UniRef100 UniRef100_P21629
UniRef50 UniRef50_P21629
UniRef90 UniRef90_P21629

Product

Feature Type CDS
Coding Frame 1
Product Name
branched-chain amino acid transport protein BraF
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.62
Kyte-Doolittle Hydrophobicity Value -0.140
Molecular Weight (kDa) 28.3
Isoelectric Point (pI) 8.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001033 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1071
Search term: braF

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 12 [Source:HGNC Symbol;Acc:HGNC:14637]
E-value: 2.0e-21
Percent Identity: 28.6

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 12 [Source:HGNC Symbol;Acc:HGNC:14637]
E-value: 2.0e-21
Percent Identity: 28.6

References

Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.
Hoshino T, Kose K
J Bacteriol 1990 Oct;172(10):5531-9
PubMed ID: 2120183