Pseudomonas aeruginosa PAO1, PA1246 (aprD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1246
Name
aprD
Replicon chromosome
Genomic location 1350747 - 1352528 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_249937.1
GI 15596443
Affymetrix PA1246_aprD_at
Entrez 881263
GenBank AAG04635.1
INSDC AAG04635.1
NCBI Locus Tag PA1246
protein_id(GenBank) gb|AAG04635.1|AE004554_3|gnl|PseudoCAP|PA1246
TIGR NTL03PA01247
UniParc UPI0000125CB5
UniProtKB Acc Q03024
UniProtKB ID APRD_PSEAE
UniRef100 UniRef100_Q03024
UniRef50 UniRef50_Q03024
UniRef90 UniRef90_Q03024

Product

Feature Type CDS
Coding Frame 1
Product Name
alkaline protease secretion protein AprD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.88
Kyte-Doolittle Hydrophobicity Value 0.221
Molecular Weight (kDa) 63.7
Isoelectric Point (pI) 9.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001198 (798 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1246
Search term: aprD

Human Homologs

Ensembl 94, assembly GRCh38.p12
ATP binding cassette subfamily B member 5 [Source:HGNC Symbol;Acc:HGNC:46]
E-value: 6.2e-34
Percent Identity: 28.5

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism.
Guzzo J, Pages JM, Duong F, Lazdunski A, Murgier M
J. Bacteriol. 1991 Sep;173(17):5290-7
PubMed ID: 1832151
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov;121(1):47-54
PubMed ID: 1427098
Protein secretion by heterologous bacterial ABC-transporters: the C-terminus secretion signal of the secreted protein confers high recognition specificity.
Duong F, Lazdunski A, Murgier M
Mol. Microbiol. 1996 Aug;21(3):459-70
PubMed ID: 8866470
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov;121(1):47-54
PubMed ID: 1427098