Pseudomonas aeruginosa PAO1, PA1248 (aprF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1248
Name
aprF
Replicon chromosome
Genomic location 1353827 - 1355272 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249939.1
GI 15596445
Affymetrix PA1248_aprF_at
Entrez 881227
GenBank AAG04637.1
INSDC AAG04637.1
NCBI Locus Tag PA1248
protein_id(GenBank) gb|AAG04637.1|AE004554_5|gnl|PseudoCAP|PA1248
TIGR NTL03PA01249
UniParc UPI0000125CB7
UniProtKB Acc Q03027
UniProtKB ID APRF_PSEAE
UniRef100 UniRef100_Q03027
UniRef50 UniRef50_Q03027
UniRef90 UniRef90_Q03027

Product

Feature Type CDS
Coding Frame 1
Product Name
Alkaline protease secretion outer membrane protein AprF precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.59
Kyte-Doolittle Hydrophobicity Value -0.531
Molecular Weight (kDa) 54.5
Isoelectric Point (pI) 6.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database Victors : 4254
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 301 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001200 (778 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1248
Search term: aprF

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov;121(1):47-54
PubMed ID: 1427098
Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
Duong F, Lazdunski A, Cami B, Murgier M
Gene 1992 Nov;121(1):47-54
PubMed ID: 1427098