Pseudomonas aeruginosa PAO1, PA1281 (cobV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1281
Name
cobV
Synonym: cobS
Replicon chromosome
Genomic location 1391864 - 1392601 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249972.1
GI 15596478
Affymetrix PA1281_cobV_at
Entrez 881439
GenBank AAG04670.1
INSDC AAG04670.1
NCBI Locus Tag PA1281
protein_id(GenBank) gb|AAG04670.1|AE004557_11|gnl|PseudoCAP|PA1281
TIGR NTL03PA01282
UniParc UPI00000C52E9
UniProtKB Acc Q9I463
UniProtKB ID COBS_PSEAE
UniRef100 UniRef100_Q9I463
UniRef50 UniRef50_Q9I463
UniRef90 UniRef90_Q9I463

Product

Feature Type CDS
Coding Frame 1
Product Name
cobalamin (5'-phosphate) synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.41
Kyte-Doolittle Hydrophobicity Value 0.937
Molecular Weight (kDa) 25.6
Isoelectric Point (pI) 9.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 343 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001228 (546 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1281
Search term: cobV

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-phosphate) synthase.
Cameron B, Blanche F, Rouyez MC, Bisch D, Famechon A, Couder M, Cauchois L, Thibaut D, Debussche L, Crouzet J
J Bacteriol 1991 Oct;173(19):6066-73
PubMed ID: 1917841