Pseudomonas aeruginosa PAO1, PA1361 (norM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1361
Name
norM
Synonym: norM
Synonym: ydhE
Synonym: pmpM
Replicon chromosome
Genomic location 1472547 - 1473980 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250052.1
GI 15596558
Affymetrix PA1361_at
Entrez 880959
GenBank AAG04750.1
INSDC AAG04750.1
NCBI Locus Tag PA1361
protein_id(GenBank) gb|AAG04750.1|AE004565_9|gnl|PseudoCAP|PA1361
TIGR NTL03PA01362
UniParc UPI0000130361
UniProtKB Acc Q9I3Y3
UniProtKB ID PMPM_PSEAE
UniRef100 UniRef100_Q9I3Y3
UniRef50 UniRef50_Q9I3Y3
UniRef90 UniRef90_Q9I3Y3

Product

Feature Type CDS
Coding Frame 1
Product Name
NorM
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 12.55
Kyte-Doolittle Hydrophobicity Value 0.632
Molecular Weight (kDa) 50.8
Isoelectric Point (pI) 9.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
PmpM PmpM is a multidrug efflux pump belonging to the MATE family of Pseudomonas aeruginosa. PmpM is an H+ drug antiporter and is the first reported case of an H+ coupled efflux pump in the MATE family. PmpM confers resistance to fluoroquinolones, fradiomycin, benzalkonium chloride, chlorhexidine gluconate, ethidium bromide, tetraphenylphosphonium chloride (TPPCl), and rhodamine 6G. ARO:3004077 protein homolog model Perfect 927.2 100.0 3.0.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 451 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001295 (997 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1361
Search term: norM
Search term: NorM

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli.
Morita Y, Kodama K, Shiota S, Mine T, Kataoka A, Mizushima T, Tsuchiya T
Antimicrob. Agents Chemother. 1998 Jul;42(7):1778-82
PubMed ID: 9661020
An H(+)-coupled multidrug efflux pump, PmpM, a member of the MATE family of transporters, from Pseudomonas aeruginosa.
He GX, Kuroda T, Mima T, Morita Y, Mizushima T, Tsuchiya T
J. Bacteriol. 2004 Jan;186(1):262-5
PubMed ID: 14679249