Pseudomonas aeruginosa PAO1, PA1409 (aphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1409
Name
aphA
Replicon chromosome
Genomic location 1533238 - 1534278 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentShown to remove acetyl group from acetyl-putrescine, acetyl-cadaverine, acetyl-spermine and acetyl-spermidine

Cross-References

RefSeq NP_250100.1
GI 15596606
Affymetrix PA1409_aphA_at
DNASU PaCD00006650
Entrez 879295
GenBank AAG04798.1
INSDC AAG04798.1
NCBI Locus Tag PA1409
protein_id(GenBank) gb|AAG04798.1|AE004570_6|gnl|PseudoCAP|PA1409
TIGR NTL03PA01410
UniParc UPI00000C5366
UniProtKB Acc Q9I3T5
UniProtKB ID Q9I3T5_PSEAE
UniRef100 UniRef100_Q9I3T5
UniRef50 UniRef50_Q9I3T5
UniRef90 UniRef90_Q9I3T5

Product

Feature Type CDS
Coding Frame 1
Product Name
acetylpolyamine aminohydrolase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -12.27
Kyte-Doolittle Hydrophobicity Value -0.231
Molecular Weight (kDa) 38.0
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 438 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001330 (480 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1409
Search term: aphA

Human Homologs

Ensembl 110, assembly GRCh38.p14
histone deacetylase 9 [Source:HGNC Symbol;Acc:HGNC:14065]
E-value: 2.8e-17
Percent Identity: 29.6
Ensembl 110, assembly GRCh38.p14
histone deacetylase 9 [Source:HGNC Symbol;Acc:HGNC:14065]
E-value: 2.8e-17
Percent Identity: 29.6
Ensembl 110, assembly GRCh38.p14
histone deacetylase 9 [Source:HGNC Symbol;Acc:HGNC:14065]
E-value: 2.8e-17
Percent Identity: 29.6
Ensembl 110, assembly GRCh38.p14
histone deacetylase 9 [Source:HGNC Symbol;Acc:HGNC:14065]
E-value: 2.8e-17
Percent Identity: 29.6
Ensembl 110, assembly GRCh38.p14
histone deacetylase 9 [Source:HGNC Symbol;Acc:HGNC:14065]
E-value: 2.8e-17
Percent Identity: 29.6

References

Crystallization and some properties of acetylpolyamine amidohydrolase from Mycoplana bullata.
Fujishiro K, Ando M, Uwajima T
Biochem Biophys Res Commun 1988 Dec 30;157(3):1169-74
PubMed ID: 3207420
Acetylpolyamine amidohydrolase from Mycoplana ramosa: gene cloning and characterization of the metal-substituted enzyme.
Sakurada K, Ohta T, Fujishiro K, Hasegawa M, Aisaka K
J Bacteriol 1996 Oct;178(19):5781-6
PubMed ID: 8824626
Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1.
Chou HT, Kwon DH, Hegazy M, Lu CD
J Bacteriol 2008 Mar;190(6):1966-75
PubMed ID: 18192388
A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe.
Meyners C, Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ
Anal Bioanal Chem 2014 Aug;406(20):4889-97
PubMed ID: 24871864
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.
Krämer A, Herzer J, Overhage J, Meyer-Almes FJ
BMC Biochem 2016 Mar 9;17:4
PubMed ID: 26956223