Pseudomonas aeruginosa PAO1, PA1443 (fliM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1443
Name
fliM
Replicon chromosome
Genomic location 1572552 - 1573523 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250134.1
GI 15596640
Affymetrix PA1443_fliM_at
Entrez 881860
GenBank AAG04832.1
INSDC AAA79753.1
NCBI Locus Tag PA1443
protein_id(GenBank) gb|AAG04832.1|AE004574_3|gnl|PseudoCAP|PA1443
TIGR NTL03PA01444
UniParc UPI000012A9D0
UniProtKB Acc Q51465
UniProtKB ID FLIM_PSEAE
UniRef100 UniRef100_Q51465
UniRef50 UniRef50_Q51465
UniRef90 UniRef90_Q51465

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar motor switch protein FliM
Product Name Confidence: Class 2
Synonyms flaii protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.82
Kyte-Doolittle Hydrophobicity Value -0.068
Molecular Weight (kDa) 36.3
Isoelectric Point (pI) 5.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Flagellar
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 292 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001360 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1443
Search term: fliM

Human Homologs

References

DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli.
Malakooti J, Komeda Y, Matsumura P
J Bacteriol 1989 May;171(5):2728-34
PubMed ID: 2651416
Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence.
Simpson DA, Ramphal R, Lory S
Infect Immun 1995 Aug;63(8):2950-7
PubMed ID: 7622217
A mutational analysis of the interaction between FliG and FliM, two components of the flagellar motor of Escherichia coli.
Marykwas DL, Berg HC
J Bacteriol 1996 Mar;178(5):1289-94
PubMed ID: 8631704
FliN is a major structural protein of the C-ring in the Salmonella typhimurium flagellar basal body.
Zhao R, Pathak N, Jaffe H, Reese TS, Khan S
J Mol Biol 1996 Aug 16;261(2):195-208
PubMed ID: 8757287