Pseudomonas aeruginosa PAO1, PA1454 (fleN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1454
Name
fleN
Synonym: motR
Replicon chromosome
Genomic location 1583956 - 1584798 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250145.1
GI 15596651
Affymetrix PA1454_at
Entrez 881866
GenBank AAG04843.1
INSDC AAG04843.1
NCBI Locus Tag PA1454
protein_id(GenBank) gb|AAG04843.1|AE004575_2|gnl|PseudoCAP|PA1454
TIGR NTL03PA01455
UniParc UPI00000D426B
UniProtKB Acc G3XD64
UniProtKB ID G3XD64_PSEAE
UniRef100 UniRef100_G3XD64
UniRef50 UniRef50_G3XD64
UniRef90 UniRef90_G3XD64

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar synthesis regulator FleN
Product Name Confidence: Class 1
Synonyms MotR
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.62
Kyte-Doolittle Hydrophobicity Value 0.196
Molecular Weight (kDa) 30.0
Isoelectric Point (pI) 8.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5JVF TRANSCRIPTION 05/11/16 Crystal Structure of Apo-FleN PSEUDOMONAS AERUGINOSA PAO1 1.66 X-RAY DIFFRACTION 100.0
5J1J TRANSCRIPTION 03/29/16 Structure of FleN-AMPPNP complex PSEUDOMONAS AERUGINOSA PAO1 1.55 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 637 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001369 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1454
Search term: fleN

Human Homologs

Ensembl 94, assembly GRCh38.p12
nucleotide binding protein 2 [Source:HGNC Symbol;Acc:HGNC:8042]
E-value: 5.0e-10
Percent Identity: 26.6

References

Activation of the multiple drug resistance gene MDR1 in fluconazole-resistant, clinical Candida albicans strains is caused by mutations in a trans-regulatory factor.
Wirsching S, Michel S, Köhler G, Morschhäuser J
J. Bacteriol. 2000 Jan;182(2):400-4
PubMed ID: 10629186
The pseudomonas aeruginosa motR gene involved in regulation of bacterial motility.
Campos-García J, Nájera R, Camarena L, Soberón-Chávez G
FEMS Microbiol. Lett. 2000 Mar;184(1):57-62
PubMed ID: 10689166
A putative ATP-binding protein from the che/fla locus of Bacillus subtilis.
Kirsch ML, Carpenter PB, Ordal GW
DNA Seq. 1994 ;4(4):271-5
PubMed ID: 7987014
The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent beta-ketoacyl reductase which is specifically involved in rhamnolipid synthesis.
Campos-García J, Caro AD, Nájera R, Miller-Maier RM, Al-Tahhan RA, Soberón-Chávez G
J. Bacteriol. 1998 Sep;180(17):4442-51
PubMed ID: 9721281