Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4446
|
Name |
algW
|
Replicon | chromosome |
Genomic location | 4979834 - 4981003 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253136.1 |
GI | 15599642 |
Affymetrix | PA4446_algW_at |
Entrez | 880976 |
GenBank | AAG07834.1 |
INSDC | AAG07834.1 |
NCBI Locus Tag | PA4446 |
protein_id(GenBank) | gb|AAG07834.1|AE004859_3|gnl|PseudoCAP|PA4446 |
TIGR | NTL03PA04447 |
UniParc | UPI00000C5D15 |
UniProtKB Acc | Q9HVX1 |
UniProtKB ID | Q9HVX1_PSEAE |
UniRef100 | UniRef100_W0YZI0 |
UniRef50 | UniRef50_G8QBT6 |
UniRef90 | UniRef90_Q02GZ5 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
AlgW protein
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -0.82 |
Kyte-Doolittle Hydrophobicity Value | 0.028 |
Molecular Weight (kDa) | 41.0 |
Isoelectric Point (pI) | 6.69 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7CO3 | PEPTIDE BINDING PROTEIN | 08/03/20 | HtrA-type protease AlgWS227A with tripeptide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT | 1.9 | X-RAY DIFFRACTION | 99.7 |
7CO7 | PEPTIDE BINDING PROTEIN | 08/04/20 | HtrA-type protease AlgWS227A with decapeptide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT | 2.6 | X-RAY DIFFRACTION | 99.7 |
7CO5 | PEPTIDE BINDING PROTEIN | 08/03/20 | HtrA-type protease AlgW with decapeptide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT | 2.345 | X-RAY DIFFRACTION | 100.0 |
7CO2 | PEPTIDE BINDING PROTEIN | 08/03/20 | HtrA-type protease AlgW with tripeptide | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT | 2.1 | X-RAY DIFFRACTION | 99.7 |
Source 1 | |
Database | Victors : 4250 |
Category | Alginate regulation |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Rattus norvegicus |
Notes | |
Infection model | Rat agar bead model of chronic lung infection |
Reference | 14641575 |
Source 2 | |
Database | VFDB |
Category | Alginate regulation |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 615 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004035 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4446
Search term: algW
Search term: AlgW protein
|
Ensembl
110, assembly
GRCh38.p14
|
HtrA serine peptidase 3 [Source:HGNC Symbol;Acc:HGNC:30406]
E-value:
2.6e-32
Percent Identity:
32.9
|
Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA.
Boucher JC, Martinez-Salazar J, Schurr MJ, Mudd MH, Yu H, Deretic V
J Bacteriol 1996 Jan;178(2):511-23
PubMed ID: 8550474
|
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc Natl Acad Sci U S A 2007 May 8;104(19):8107-12
PubMed ID: 17470813
|
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J Bacteriol 2009 Apr;191(7):2285-95
PubMed ID: 19168621
|