Pseudomonas aeruginosa PAO1, PA4446 (algW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4446
Name
algW
Replicon chromosome
Genomic location 4979834 - 4981003 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253136.1
GI 15599642
Affymetrix PA4446_algW_at
Entrez 880976
GenBank AAG07834.1
INSDC AAG07834.1
NCBI Locus Tag PA4446
protein_id(GenBank) gb|AAG07834.1|AE004859_3|gnl|PseudoCAP|PA4446
TIGR NTL03PA04447
UniParc UPI00000C5D15
UniProtKB Acc Q9HVX1
UniProtKB ID Q9HVX1_PSEAE
UniRef100 UniRef100_W0YZI0
UniRef50 UniRef50_G8QBT6
UniRef90 UniRef90_Q02GZ5

Product

Feature Type CDS
Coding Frame 1
Product Name
AlgW protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.82
Kyte-Doolittle Hydrophobicity Value 0.028
Molecular Weight (kDa) 41.0
Isoelectric Point (pI) 6.69

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB
Category Alginate regulation
Evidence
Host Organism
Notes
Reference
Source 2
Database Victors : 4250
Category Alginate regulation
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 615 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004035 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4446
Search term: algW
Search term: AlgW protein

Human Homologs

Ensembl 97, assembly GRCh38.p12
HtrA serine peptidase 3 [Source:HGNC Symbol;Acc:HGNC:30406]
E-value: 2.3e-32
Percent Identity: 32.9

References

Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA.
Boucher JC, Martinez-Salazar J, Schurr MJ, Mudd MH, Yu H, Deretic V
J. Bacteriol. 1996 Jan;178(2):511-23
PubMed ID: 8550474
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J. Bacteriol. 2009 Apr;191(7):2285-95
PubMed ID: 19168621