Pseudomonas aeruginosa PAO1, PA4460 (lptH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4460
Name
lptH
Synonym: yhbN
Replicon chromosome
Genomic location 4991392 - 4991919 (+ strand)

Cross-References

RefSeq NP_253150.1
GI 15599656
Affymetrix PA4460_at
DNASU PaCD00007934
Entrez 881017
GenBank AAG07848.1
INSDC AAG07848.1
NCBI Locus Tag PA4460
protein_id(GenBank) gb|AAG07848.1|AE004860_4|gnl|PseudoCAP|PA4460
TIGR NTL03PA04461
UniParc UPI00000C5D1F
UniProtKB Acc Q9HVV7
UniProtKB ID Q9HVV7_PSEAE
UniRef100 UniRef100_W0YZJ5
UniRef50 UniRef50_A6VBD4
UniRef90 UniRef90_A6VBD4

Product

Feature Type CDS
Coding Frame 1
Product Name
LptH
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.99
Kyte-Doolittle Hydrophobicity Value -0.358
Molecular Weight (kDa) 19.1
Isoelectric Point (pI) 9.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4UU4 TRANSPORT PROTEIN 07/24/14 Crystal structure of LptH, the LptA homologous periplasmic component of the conserved lipopolysaccharide transport device from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.751 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 131 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004049 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4460
Search term: lptH
Search term: LptH

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Crystal structure of LptH, the periplasmic component of the lipopolysaccharide transport machinery from Pseudomonas aeruginosa.
Bollati M, Villa R, Gourlay LJ, Benedet M, Dehò G, Polissi A, Barbiroli A, Martorana AM, Sperandeo P, Bolognesi M, Nardini M
FEBS J. 2015 May;282(10):1980-97
PubMed ID: 25735820
Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli.
Okuda S, Freinkman E, Kahne D
Science 2012 Nov;338(6111):1214-7
PubMed ID: 23138981