Pseudomonas aeruginosa PAO1, PA4461 (lptB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4461
Name
lptB
Synonym: yhbG
Replicon chromosome
Genomic location 4991919 - 4992644 (+ strand)

Cross-References

RefSeq NP_253151.1
GI 15599657
Affymetrix PA4461_at
DNASU PaCD00007935
Entrez 881018
GenBank AAG07849.1
INSDC AAG07849.1
NCBI Locus Tag PA4461
protein_id(GenBank) gb|AAG07849.1|AE004860_5|gnl|PseudoCAP|PA4461
TIGR NTL03PA04462
UniParc UPI00000C5D20
UniProtKB Acc Q9HVV6
UniProtKB ID Q9HVV6_PSEAE
UniRef100 UniRef100_W0YWK0
UniRef50 UniRef50_P45073
UniRef90 UniRef90_A0A0B7D3U1

Product

Feature Type CDS
Coding Frame 1
Product Name
Lipopolysaccharide export system ATP-binding protein LptB
Product Name Confidence: Class 2
Synonyms probable ATP-binding component of ABC transporter
Evidence for Translation
Detected using LC-MS/MS (26.45 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.66
Kyte-Doolittle Hydrophobicity Value -0.070
Molecular Weight (kDa) 26.5
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5X5Y MEMBRANE PROTEIN 02/18/17 A membrane protein complex PSEUDOMONAS AERUGINOSA PAO1 3.465 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004050 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4461
Search term: lptB

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 13 [Source:HGNC Symbol;Acc:HGNC:14638]
E-value: 8.5e-22
Percent Identity: 29.4

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 13 [Source:HGNC Symbol;Acc:HGNC:14638]
E-value: 8.5e-22
Percent Identity: 29.4

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATP binding cassette subfamily A member 13 [Source:HGNC Symbol;Acc:HGNC:14638]
E-value: 8.5e-22
Percent Identity: 29.4

References

The requirement of RpoN (sigma factor sigma54) in denitrification by Pseudomonas stutzeri is indirect and restricted to the reduction of nitrite and nitric oxide.
Härtig E, Zumft WG
Appl. Environ. Microbiol. 1998 Aug;64(8):3092-5
PubMed ID: 9687481
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli.
Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Dehò G, Polissi A
J. Bacteriol. 2007 Jan;189(1):244-53
PubMed ID: 17056748