Pseudomonas aeruginosa PAO1, PA5324 (sphR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5324
Name
sphR
Replicon chromosome
Genomic location 5994741 - 5995811 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentBinds sphingosine and induces expression of sphA (PA5325), sphBCD (PA5328-PA5326), and cerN (PA0845)

Cross-References

RefSeq NP_254011.1
GI 15600517
Affymetrix PA5324_at
Entrez 879621
GenBank AAG08709.1
INSDC AAG08709.1
NCBI Locus Tag PA5324
protein_id(GenBank) gb|AAG08709.1|AE004945_3|gnl|PseudoCAP|PA5324
TIGR NTL03PA05325
UniParc UPI00000C5FA5
UniProtKB Acc Q9HTN1
UniProtKB ID Q9HTN1_PSEAE
UniRef100 UniRef100_W0Z282
UniRef50 UniRef50_Q4KAC5
UniRef90 UniRef90_W0Z282

Product

Feature Type CDS
Coding Frame 1
Product Name
Sphingosine-responsive Regulator, SphR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 3.77
Kyte-Doolittle Hydrophobicity Value -0.085
Molecular Weight (kDa) 39.8
Isoelectric Point (pI) 8.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 228 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000800 (1482 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5324
Search term: sphR

Human Homologs

References

Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog 2014 Jan;10(1):e1003889
PubMed ID: 24465209