Pseudomonas aeruginosa PAO1, PA5398 (dgcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5398
Name
dgcA
Synonym: dgcA
Replicon chromosome
Genomic location 6075880 - 6077940 (+ strand)
Transposon Mutants 12 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStrains with deletions in dgcA are unable to grow on choline, glycine betaine, or dimethylglcycine but are able to utilize sarcosine. NMR analysis shows buildup of labeled dimethylglycine when dgcA mutants are given labeled choline. Presumed to function with DgcB (PA5399).

Cross-References

RefSeq NP_254085.1
GI 15600591
Affymetrix PA5398_at
Entrez 878122
GenBank AAG08783.1
INSDC AAG08783.1
NCBI Locus Tag PA5398
protein_id(GenBank) gb|AAG08783.1|AE004952_5|gnl|PseudoCAP|PA5398
TIGR NTL03PA05399
UniParc UPI00000C5FE3
UniProtKB Acc Q9HTG6
UniProtKB ID Q9HTG6_PSEAE
UniRef100 UniRef100_Q9HTG6
UniRef50 UniRef50_Q9HTG6
UniRef90 UniRef90_Q9HTG6

Product

Feature Type CDS
Coding Frame 1
Product Name
DgcA, Dimethylglycine catabolism
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -13.28
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 75.7
Isoelectric Point (pI) 5.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 509 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004955 (527 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5398
Search term: dgcA

References

Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379