Pseudomonas aeruginosa PAO1, PA5410 (gbcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5410
Name
gbcA
Synonym: gbcA
Replicon chromosome
Genomic location 6085385 - 6086674 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentStrains with deletions in gbcA are unable to grow on choline or glycine betaine but are able to grow on dimethylglycine. NMR analysis shows buildup of labeled glycine betaine when gbcA mutants are givenlabeled choline.

Cross-References

RefSeq NP_254097.1
GI 15600603
Affymetrix PA5410_at
Entrez 880240
GenBank AAG08795.1
INSDC AAG08795.1
NCBI Locus Tag PA5410
protein_id(GenBank) gb|AAG08795.1|AE004953_9|gnl|PseudoCAP|PA5410
TIGR NTL03PA05411
UniParc UPI00000C5FEF
UniProtKB Acc Q9HTF4
UniProtKB ID Q9HTF4_PSEAE
UniRef100 UniRef100_W0Z2H4
UniRef50 UniRef50_W9VLP0
UniRef90 UniRef90_A0A024HQV1

Product

Feature Type CDS
Coding Frame 1
Product Name
GbcA
Product Name Confidence: Class 1
Synonyms glycine betaine catabolism protein
GbcA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.41
Kyte-Doolittle Hydrophobicity Value -0.385
Molecular Weight (kDa) 48.7
Isoelectric Point (pI) 6.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 192 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003046 (1343 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5410
Search term: gbcA
Search term: GbcA

References

Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379