Pseudomonas aeruginosa PAO1, PA5411 (gbcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5411
Name
gbcB
Synonym: gbcB
Replicon chromosome
Genomic location 6087099 - 6088199 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStrains with deletions in gbcB are unable to grow on choline or glycine betaine but are able to grow on dimethylglycine. NMR analysis shows buildup of labeled glycine betaine when gbcB mutants are given labeled choline. Presumed to function with GbcA (PA5410).

Cross-References

RefSeq NP_254098.1
GI 15600604
Affymetrix PA5411_at
Entrez 880070
GenBank AAG08796.1
INSDC AAG08796.1
NCBI Locus Tag PA5411
protein_id(GenBank) gb|AAG08796.1|AE004953_10|gnl|PseudoCAP|PA5411
TIGR NTL03PA05412
UniParc UPI00000C5FF0
UniProtKB Acc Q9HTF3
UniProtKB ID Q9HTF3_PSEAE
UniRef100 UniRef100_W0Z1S6
UniRef50 UniRef50_G8Q2E0
UniRef90 UniRef90_W6RKV2

Product

Feature Type CDS
Coding Frame 1
Product Name
GbcB
Product Name Confidence: Class 1
Synonyms GbcB
glycine betaine catabolism protein
Evidence for Translation
Charge (pH 7) -10.69
Kyte-Doolittle Hydrophobicity Value -0.063
Molecular Weight (kDa) 40.5
Isoelectric Point (pI) 5.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 486 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004967 (539 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5411
Search term: gbcB
Search term: GbcB

References

Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379