Pseudomonas aeruginosa PAO1, PA5414

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5414
Name
Replicon chromosome
Genomic location 6093167 - 6093808 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254101.1
GI 15600607
Affymetrix PA5414_at
Entrez 880065
GenBank AAG08799.1
INSDC AAG08799.1
NCBI Locus Tag PA5414
protein_id(GenBank) gb|AAG08799.1|AE004954_1|gnl|PseudoCAP|PA5414
TIGR NTL03PA05415
UniParc UPI00000C5FF2
UniProtKB Acc Q9HTF0
UniProtKB ID Q9HTF0_PSEAE
UniRef100 UniRef100_W0YZI8
UniRef50 UniRef50_A6VEM8
UniRef90 UniRef90_Q02DU7

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (22.55 kDa, PMID:21751344)
Charge (pH 7) -1.62
Kyte-Doolittle Hydrophobicity Value -0.142
Molecular Weight (kDa) 22.5
Isoelectric Point (pI) 5.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004969 (305 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5414

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344