Pseudomonas aeruginosa PAO1, PA5416 (soxB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5416
Name
soxB
Replicon chromosome
Genomic location 6095363 - 6096613 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentStrains with transposon mutants in any of the soxBDAG genes are unable to grow on choline, glycine betaine, dimethylglcycine or sarcosine.

Cross-References

RefSeq NP_254103.1
GI 15600609
Affymetrix PA5416_soxB_at
DNASU PaCD00008339
Entrez 880060
GenBank AAG08801.1
INSDC AAG08801.1
NCBI Locus Tag PA5416
protein_id(GenBank) gb|AAG08801.1|AE004954_3|gnl|PseudoCAP|PA5416
TIGR NTL03PA05417
UniParc UPI00000C5FF3
UniProtKB Acc Q9HTE8
UniProtKB ID Q9HTE8_PSEAE
UniRef100 UniRef100_Q9HTE8
UniRef50 UniRef50_O87388
UniRef90 UniRef90_Q9HTE8

Product

Feature Type CDS
Coding Frame 1
Product Name
sarcosine oxidase beta subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 4.72
Kyte-Doolittle Hydrophobicity Value -0.180
Molecular Weight (kDa) 45.1
Isoelectric Point (pI) 8.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 323 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004970 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5416
Search term: soxB

Human Homologs

Ensembl 94, assembly GRCh38.p12
dimethylglycine dehydrogenase [Source:HGNC Symbol;Acc:HGNC:24475]
E-value: 2.1e-12
Percent Identity: 22.5

References

Cysteine residues in the active site of Corynebacterium sarcosine oxidase.
Suzuki H, Kawamura-Konishi Y
J. Biochem. 1991 Jun;109(6):909-17
PubMed ID: 1939012
Preparation and properties of recombinant corynebacterial sarcosine oxidase: evidence for posttranslational modification during turnover with sarcosine.
Chlumsky LJ, Zhang L, Ramsey AJ, Jorns MS
Biochemistry 1993 Oct;32(41):11132-42
PubMed ID: 7692961
Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379