Pseudomonas aeruginosa PAO1, PA5427 (adhA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5427
Name
adhA
Replicon chromosome
Genomic location 6107111 - 6108139 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254114.1
GI 15600620
Affymetrix PA5427_adhA_at
DNASU PaCD00008344
Entrez 877625
GenBank AAG08812.1
INSDC AAG08812.1
NCBI Locus Tag PA5427
protein_id(GenBank) gb|AAG08812.1|AE004955_6|gnl|PseudoCAP|PA5427
TIGR NTL03PA05428
UniParc UPI00000C5FFC
UniProtKB Acc Q9HTD9
UniProtKB ID Q9HTD9_PSEAE
UniRef100 UniRef100_W0Z1V1
UniRef50 UniRef50_F9DQT4
UniRef90 UniRef90_W0Z1V1

Product

Feature Type CDS
Coding Frame 1
Product Name
alcohol dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (35.91 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.81
Kyte-Doolittle Hydrophobicity Value 0.165
Molecular Weight (kDa) 35.9
Isoelectric Point (pI) 5.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1LLU OXIDOREDUCTASE 04/30/02 THE TERNARY COMPLEX OF PSEUDOMONAS AERUGINOSA ALCOHOL DEHYDROGENASE WITH ITS COENZYME AND WEAK SUBSTRATE PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 578 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004980 (431 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5427
Search term: adhA

References

A few amino acid substitutions are responsible for the higher thermostability of a novel NAD(+)-dependent bacillar alcohol dehydrogenase.
Cannio R, Rossi M, Bartolucci S
Eur. J. Biochem. 1994 Jun;222(2):345-52
PubMed ID: 8020473
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344