Pseudomonas aeruginosa PAO1, PA5442

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5442
Name
Replicon chromosome
Genomic location 6128056 - 6130911 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentOverexpression of PA5442 impairs TTSS-mediated cytotoxicity

Cross-References

RefSeq NP_254129.1
GI 15600635
Affymetrix PA5442_at
Entrez 877848
GenBank AAG08827.1
INSDC AAG08827.1
NCBI Locus Tag PA5442
protein_id(GenBank) gb|AAG08827.1|AE004957_2|gnl|PseudoCAP|PA5442
TIGR NTL03PA05443
UniParc UPI00000C600B
UniProtKB Acc Q9HTC4
UniProtKB ID Q9HTC4_PSEAE
UniRef100 UniRef100_Q9HTC4
UniRef50 UniRef50_Q4K3G4
UniRef90 UniRef90_Q9HTC4

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.43
Kyte-Doolittle Hydrophobicity Value -0.087
Molecular Weight (kDa) 106.4
Isoelectric Point (pI) 7.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 521 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004258 (1005 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5442

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295