Pseudomonas aeruginosa PAO1, PA5458

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5458
Name
Replicon chromosome
Genomic location 6149179 - 6150321 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentAn in-frame deletion knockout mutant has a shift in banding pattern of LPS with common polysaccharide (also called A-band) when the LPS prepared from the mutant was analyzed by SDS-PAGE and silver staining, as well by Western blotting and probed with monoclonal antibody N1F10 (specific against A-band LPS).

Cross-References

RefSeq NP_254145.1
GI 15600651
Affymetrix PA5458_at
Entrez 879496
GenBank AAG08843.1
INSDC AAG08843.1
NCBI Locus Tag PA5458
protein_id(GenBank) gb|AAG08843.1|AE004958_13|gnl|PseudoCAP|PA5458
TIGR NTL03PA05459
UniParc UPI00000C6017
UniProtKB Acc Q9HTB2
UniProtKB ID Q9HTB2_PSEAE
UniRef100 UniRef100_Q9HTB2
UniRef50 UniRef50_Q9HTB2
UniRef90 UniRef90_Q9HTB2

Product

Feature Type CDS
Coding Frame 1
Product Name
putative acetyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) 15.73
Kyte-Doolittle Hydrophobicity Value 0.667
Molecular Weight (kDa) 42.4
Isoelectric Point (pI) 10.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 220 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005004 (408 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5458

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Five new genes are important for common polysaccharide antigen biosynthesis in Pseudomonas aeruginosa.
Hao Y, King JD, Huszczynski S, Kocíncová D, Lam JS
mBio 2013 Jan 22;4(1):e00631-12
PubMed ID: 23341552