Pseudomonas aeruginosa PAO1, PA5474

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5474
Name
Replicon chromosome
Genomic location 6164038 - 6165411 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254161.1
GI 15600667
Affymetrix PA5474_at
Entrez 880371
GenBank AAG08859.1
INSDC AAG08859.1
NCBI Locus Tag PA5474
protein_id(GenBank) gb|AAG08859.1|AE004960_2|gnl|PseudoCAP|PA5474
TIGR NTL03PA05475
UniParc UPI00000C6027
UniProtKB Acc Q9HT96
UniProtKB ID Q9HT96_PSEAE
UniRef100 UniRef100_Q9HT96
UniRef50 UniRef50_Q4KKH9
UniRef90 UniRef90_Q9HT96

Product

Feature Type CDS
Coding Frame 1
Product Name
probable metalloprotease
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.45
Kyte-Doolittle Hydrophobicity Value -0.304
Molecular Weight (kDa) 51.4
Isoelectric Point (pI) 4.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 344 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005016 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5474

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295