Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5485
|
Name |
ampDh2
|
Replicon | chromosome |
Genomic location | 6176516 - 6177295 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_254172.1 |
GI | 15600678 |
Affymetrix | PA5485_at |
DNASU | PaCD00008362 |
Entrez | 880851 |
GenBank | AAG08870.1 |
INSDC | AAG08870.1 |
NCBI Locus Tag | PA5485 |
protein_id(GenBank) | gb|AAG08870.1|AE004961_5|gnl|PseudoCAP|PA5485 |
TIGR | NTL03PA05486 |
UniParc | UPI00000C6030 |
UniProtKB Acc | Q9HT86 |
UniProtKB ID | Q9HT86_PSEAE |
UniRef100 | UniRef100_W0Z228 |
UniRef50 | UniRef50_Q4KKJ3 |
UniRef90 | UniRef90_W0Z228 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
AmpDh2
|
Synonyms |
AmpDh2 (changed from conserved hypothetical protein) |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 0.89 |
Kyte-Doolittle Hydrophobicity Value | -0.296 |
Molecular Weight (kDa) | 28.9 |
Isoelectric Point (pI) | 7.29 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4BJ4 | HYDROLASE | 04/16/13 | Structure of Pseudomonas aeruginosa amidase Ampdh2 | PSEUDOMONAS AERUGINOSA PAO1 | 1.722 | X-RAY DIFFRACTION | 100.0 |
4BOL | HYDROLASE | 05/21/13 | Crystal structure of AmpDh2 from Pseudomonas aeruginosa in complex with pentapeptide | PSEUDOMONAS AERUGINOSA PAO1 | 1.7 | X-RAY DIFFRACTION | 99.6 |
4BPA | HYDROLASE | 05/23/13 | Crystal structure of AmpDh2 from Pseudomonas aeruginosa in complex with NAG-NAM-NAG-NAM tetrasaccharide | PSEUDOMONAS AERUGINOSA PAO1 | 2.7 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 249 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG005025 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5485
Search term: ampDh2
Search term: AmpDh2
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Stepwise upregulation of the Pseudomonas aeruginosa chromosomal cephalosporinase conferring high-level beta-lactam resistance involves three AmpD homologues.
Juan C, Moyá B, Pérez JL, Oliver A
Antimicrob Agents Chemother 2006 May;50(5):1780-7
PubMed ID: 16641450
|