Pseudomonas aeruginosa PAO1, PA5487 (dgcH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5487
Name
dgcH
Replicon chromosome
Genomic location 6178036 - 6180051 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentOverexpression PA5487 increases pellicle formation

Cross-References

RefSeq NP_254174.1
GI 15600680
Affymetrix PA5487_at
Entrez 877705
GenBank AAG08872.1
INSDC AAG08872.1
NCBI Locus Tag PA5487
protein_id(GenBank) gb|AAG08872.1|AE004961_7|gnl|PseudoCAP|PA5487
TIGR NTL03PA05488
UniParc UPI00000C6032
UniProtKB Acc Q9HT84
UniProtKB ID Q9HT84_PSEAE
UniRef100 UniRef100_W0Z3V0
UniRef50 UniRef50_B1JFI8
UniRef90 UniRef90_W0Z3V0

Product

Feature Type CDS
Coding Frame 1
Product Name
DgcH
Product Name Confidence: Class 1
Synonyms diguanylate cyclase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -32.88
Kyte-Doolittle Hydrophobicity Value -0.480
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 4.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 455 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005027 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5487
Search term: dgcH
Search term: DgcH

Human Homologs

References

Tyrosine Phosphatase TpbA of Pseudomonas aeruginosa Controls Extracellular DNA via Cyclic Diguanylic Acid Concentrations.
Ueda A, Wood TK
Environ Microbiol 2010 Jun;2(3):449-55
PubMed ID: 21552365
Diguanylate Cyclases and Phosphodiesterases Required for Basal-Level c-di-GMP in Pseudomonas aeruginosa as Revealed by Systematic Phylogenetic and Transcriptomic Analyses.
Wei Q, Leclercq S, Bhasme P, Xu A, Zhu B, Zhang Y, Zhang M, Wang S, Ma LZ
Appl Environ Microbiol 2019 Nov 1;85(21)
PubMed ID: 31444209