Pseudomonas aeruginosa PAO1, PA5497 (nrdJa)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5497
Name
nrdJa
Replicon chromosome
Genomic location 6188872 - 6191076 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentnrdJa and nrdJb are the subunits that form a functional Class II ribonucleotide reductase

Cross-References

RefSeq NP_254184.1
GI 15600690
Affymetrix PA5497_at
Entrez 877840
GenBank AAG08882.1
INSDC AAG08882.1
NCBI Locus Tag PA5497
protein_id(GenBank) gb|AAG08882.1|AE004962_6|gnl|PseudoCAP|PA5497
TIGR NTL03PA05498
UniParc UPI00000C6038
UniProtKB Acc Q9HT76
UniProtKB ID Q9HT76_PSEAE
UniRef100 UniRef100_Q9HT76
UniRef50 UniRef50_U2E6Q5
UniRef90 UniRef90_A6VEX4

Product

Feature Type CDS
Coding Frame 1
Product Name
class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJa
Product Name Confidence: Class 1
Synonyms class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJa
NrdJa (changed from hypothetical protein)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.17
Kyte-Doolittle Hydrophobicity Value -0.453
Molecular Weight (kDa) 82.7
Isoelectric Point (pI) 5.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 579 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005036 (340 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5497
Search term: nrdJa

References

Two proteins mediate class II ribonucleotide reductase activity in Pseudomonas aeruginosa: expression and transcriptional analysis of the aerobic enzymes.
Torrents E, Poplawski A, Sjöberg BM
J Biol Chem 2005 Apr 29;280(17):16571-8
PubMed ID: 15722359