Pseudomonas aeruginosa PAO1, PA5512 (mifS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5512
Name
mifS
Replicon chromosome
Genomic location 6204444 - 6206210 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254199.1
GI 15600705
Affymetrix PA5512_at
Entrez 877988
GenBank AAG08897.1
INSDC AAG08897.1
NCBI Locus Tag PA5512
protein_id(GenBank) gb|AAG08897.1|AE004963_10|gnl|PseudoCAP|PA5512
TIGR NTL03PA05513
UniParc UPI00000C6047
UniProtKB Acc Q9HT61
UniProtKB ID Q9HT61_PSEAE
UniRef100 UniRef100_Q9HT61
UniRef50 UniRef50_Q4K7A0
UniRef90 UniRef90_Q9HT61

Product

Feature Type CDS
Coding Frame 1
Product Name
MifS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.08
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 65.2
Isoelectric Point (pI) 6.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 607 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005050 (545 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5512
Search term: mifS
Search term: MifS

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic analysis and regulation of the Rhizobium meliloti genes controlling C4-dicarboxylic acid transport.
Wang YP, Birkenhead K, Boesten B, Manian S, O'Gara F
Gene 1989 Dec 21;85(1):135-44
PubMed ID: 2695394
Analysis of the C4-dicarboxylate transport genes of Rhizobium meliloti: nucleotide sequence and deduced products of dctA, dctB, and dctD.
Watson RJ
Mol. Plant Microbe Interact. ;3(3):174-81
PubMed ID: 2134335
A novel signaling network essential for regulating Pseudomonas aeruginosa biofilm development.
Petrova OE, Sauer K
PLoS Pathog. 2009 Nov;5(11):e1000668
PubMed ID: 19936057