Pseudomonas aeruginosa PAO1, PA5514

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5514
Name
Replicon chromosome
Genomic location 6207241 - 6208029 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254201.1
GI 15600707
Affymetrix PA5514_at
Entrez 877990
GenBank AAG08899.1
INSDC AAG08899.1
NCBI Locus Tag PA5514
protein_id(GenBank) gb|AAG08899.1|AE004964_2|gnl|PseudoCAP|PA5514
TIGR NTL03PA05515
UniParc UPI00000C6049
UniProtKB Acc G3XCV9
UniProtKB ID G3XCV9_PSEAE
UniRef100 UniRef100_Q6PLK5
UniRef50 UniRef50_Q6JTT6
UniRef90 UniRef90_Q6JTT6

Product

Feature Type CDS
Coding Frame 1
Product Name
probable beta-lactamase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Charge (pH 7) 5.90
Kyte-Doolittle Hydrophobicity Value -0.185
Molecular Weight (kDa) 29.1
Isoelectric Point (pI) 9.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
OXA-50 OXA-50 is a beta-lactamase found in Pseudomonas aeruginosa. It confers decreased susceptibility to ampicillin and ticarcillin and, interestingly, to moxalactam and meropenem in P. aeruginosa but not in E. coli. Also confers resistance to piperacillin-tazobactam and cephalotin. ARO:3001796 protein homolog model Perfect 542.0 100.0 3.0.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 90 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005052 (306 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5514

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Phylogeny of LCR-1 and OXA-5 with class A and class D beta-lactamases.
Couture F, Lachapelle J, Levesque RC
Mol. Microbiol. 1992 Jun;6(12):1693-705
PubMed ID: 1495394