Pseudomonas aeruginosa PAO1, PA5516 (pdxY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5516
Name
pdxY
Replicon chromosome
Genomic location 6208575 - 6209441 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254203.1
GI 15600709
Affymetrix PA5516_pdxY_at
DNASU PaCD00008376
Entrez 878628
GenBank AAG08901.1
INSDC AAG08901.1
NCBI Locus Tag PA5516
protein_id(GenBank) gb|AAG08901.1|AE004964_4|gnl|PseudoCAP|PA5516
TIGR NTL03PA05517
UniParc UPI00000C604B
UniProtKB Acc Q9HT57
UniProtKB ID PDXY_PSEAE
UniRef100 UniRef100_Q9HT57
UniRef50 UniRef50_P77150
UniRef90 UniRef90_Q9HT57

Product

Feature Type CDS
Coding Frame 1
Product Name
pyridoxamine kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.05
Kyte-Doolittle Hydrophobicity Value 0.108
Molecular Weight (kDa) 31.3
Isoelectric Point (pI) 6.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5B6A TRANSFERASE 05/25/16 Structure of Pyridoxal Kinasefrom Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 138 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005054 (511 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5516
Search term: pdxY

Human Homologs

Ensembl 94, assembly GRCh38.p12
pyridoxal kinase [Source:HGNC Symbol;Acc:HGNC:8819]
E-value: 9.7e-33
Percent Identity: 28.7

References

Identification and function of the pdxY gene, which encodes a novel pyridoxal kinase involved in the salvage pathway of pyridoxal 5'-phosphate biosynthesis in Escherichia coli K-12.
Yang Y, Tsui HC, Man TK, Winkler ME
J. Bacteriol. 1998 Apr;180(7):1814-21
PubMed ID: 9537380