Pseudomonas aeruginosa PAO1, PA5545

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5545
Name
Replicon chromosome
Genomic location 6238325 - 6239284 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentStructural similarity to 1US5 ( putative glutamate/glutamine-binding protein from Thermus thermophilus, root-mean-square deviation: 0.5 angstroms). Sequence similarity to 1US5 (99%/41,83%) (GLU). Final predicted ligand: glutamic Acid, glutamine (PMID: 31631799)

Cross-References

RefSeq NP_254232.1
GI 15600738
Affymetrix PA5545_at
DNASU PaCD00007312
Entrez 879590
GenBank AAG08930.1
INSDC AAG08930.1
NCBI Locus Tag PA5545
protein_id(GenBank) gb|AAG08930.1|AE004967_1|gnl|PseudoCAP|PA5545
TIGR NTL03PA05546
UniParc UPI00000C6067
UniProtKB Acc Q9HT29
UniProtKB ID Q9HT29_PSEAE
UniRef100 UniRef100_Q9HT29
UniRef50 UniRef50_G8Q9I4
UniRef90 UniRef90_Q9HT29

Product

Feature Type CDS
Coding Frame 1
Product Name
putative glutamate/glutamine-binding protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.19
Kyte-Doolittle Hydrophobicity Value 0.088
Molecular Weight (kDa) 33.6
Isoelectric Point (pI) 7.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 212 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000184 (763 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5545

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799