Pseudomonas aeruginosa PAO1, PA5549 (glmS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5549
Name
glmS
Replicon chromosome
Genomic location 6243111 - 6244946 (- strand)

Cross-References

RefSeq NP_254236.1
GI 15600742
Affymetrix PA5549_glmS_at
Entrez 877797
GenBank AAG08934.1
INSDC AAG08934.1
NCBI Locus Tag PA5549
protein_id(GenBank) gb|AAG08934.1|AE004967_5|gnl|PseudoCAP|PA5549
TIGR NTL03PA05550
UniParc UPI0000165EE0
UniProtKB Acc Q9HT25
UniProtKB ID GLMS_PSEAE
UniRef100 UniRef100_Q9HT25
UniRef50 UniRef50_Q9HT25
UniRef90 UniRef90_Q9HT25

Product

Feature Type CDS
Coding Frame 1
Product Name
glucosamine--fructose-6-phosphate aminotransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.09
Kyte-Doolittle Hydrophobicity Value -0.012
Molecular Weight (kDa) 66.3
Isoelectric Point (pI) 5.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 649 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005080 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5549
Search term: glmS

Human Homologs

Ensembl 97, assembly GRCh38.p12
glutamine-fructose-6-phosphate transaminase 2 [Source:HGNC Symbol;Acc:HGNC:4242]
E-value: 2.3e-108
Percent Identity: 38.2

References

Molecular cloning and overexpression of the glucosamine synthetase gene from Escherichia coli.
Dutka-Malen S, Mazodier P, Badet B
Biochimie 1988 Feb;70(2):287-90
PubMed ID: 3134953
Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 A crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase.
Isupov MN, Obmolova G, Butterworth S, Badet-Denisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A
Structure 1996 Jul;4(7):801-10
PubMed ID: 8805567
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994