Pseudomonas aeruginosa PAO1, PA5552 (glmU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5552
Name
glmU
Synonym: gcaD
Replicon chromosome
Genomic location 6246326 - 6247690 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254239.1
GI 15600745
Affymetrix PA5552_glmU_at
Entrez 877892
GenBank AAG08937.1
INSDC AAG08937.1
NCBI Locus Tag PA5552
protein_id(GenBank) gb|AAG08937.1|AE004967_8|gnl|PseudoCAP|PA5552
TIGR NTL03PA05553
UniParc UPI00000C606D
UniProtKB Acc Q9HT22
UniProtKB ID GLMU_PSEAE
UniRef100 UniRef100_Q9HT22
UniRef50 UniRef50_Q8PGH2
UniRef90 UniRef90_Q9HT22

Product

Feature Type CDS
Coding Frame 1
Product Name
glucosamine-1-phosphate acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase
Product Name Confidence: Class 2
Synonyms UDP-N-acetylglucosamine pyrophosphorylase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.46
Kyte-Doolittle Hydrophobicity Value -0.114
Molecular Weight (kDa) 48.9
Isoelectric Point (pI) 6.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 614 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005083 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5552
Search term: glmU

References

Identification of the glmU gene encoding N-acetylglucosamine-1-phosphate uridyltransferase in Escherichia coli.
Mengin-Lecreulx D, van Heijenoort J
J. Bacteriol. 1993 Oct;175(19):6150-7
PubMed ID: 8407787
Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis.
Mengin-Lecreulx D, van Heijenoort J
J. Bacteriol. 1994 Sep;176(18):5788-95
PubMed ID: 8083170
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994