Pseudomonas aeruginosa PAO1, PA5556 (atpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5556
Name
atpA
Synonym: uncA
Synonym: papA
Replicon chromosome
Genomic location 6250596 - 6252140 (- strand)

Cross-References

RefSeq NP_254243.1
GI 15600749
Affymetrix PA5556_atpA_at
Entrez 877752
GenBank AAG08941.1
INSDC AAG08941.1
NCBI Locus Tag PA5556
protein_id(GenBank) gb|AAG08941.1|AE004967_12|gnl|PseudoCAP|PA5556
TIGR NTL03PA05557
UniParc UPI00000C6070
UniProtKB Acc Q9HT18
UniProtKB ID ATPA_PSEAE
UniRef100 UniRef100_A6VF34
UniRef50 UniRef50_P92549
UniRef90 UniRef90_Q87TT2

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP synthase alpha chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (55.39 kDa, PMID:21751344)
Charge (pH 7) -7.35
Kyte-Doolittle Hydrophobicity Value -0.073
Molecular Weight (kDa) 55.4
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 666 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005087 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5556
Search term: atpA

Human Homologs

Ensembl 110, assembly GRCh38.p14
ATP synthase F1 subunit alpha [Source:HGNC Symbol;Acc:HGNC:823]
E-value: 3.1e-158
Percent Identity: 56.4
Ensembl 110, assembly GRCh38.p14
ATP synthase F1 subunit alpha [Source:HGNC Symbol;Acc:HGNC:823]
E-value: 3.1e-158
Percent Identity: 56.4

References

Nucleotide sequence of the genes coding for alpha, beta and gamma subunits of the proton-translocating ATPase of Escherichia coli.
Kanazawa H, Kayano T, Mabuchi K, Futai M
Biochem Biophys Res Commun 1981 Nov 30;103(2):604-12
PubMed ID: 6277310
Directed mutagenesis of the strongly conserved lysine 175 in the proposed nucleotide-binding domain of alpha-subunit from Escherichia coli F1-ATPase.
Rao R, Pagan J, Senior AE
J Biol Chem 1988 Nov 5;263(31):15957-63
PubMed ID: 2903146
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344