Pseudomonas aeruginosa PAO1, PA5556 (atpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5556
Name
atpA
Synonym: uncA
Synonym: papA
Replicon chromosome
Genomic location 6250596 - 6252140 (- strand)

Cross-References

RefSeq NP_254243.1
GI 15600749
Affymetrix PA5556_atpA_at
Entrez 877752
GenBank AAG08941.1
INSDC AAG08941.1
NCBI Locus Tag PA5556
protein_id(GenBank) gb|AAG08941.1|AE004967_12|gnl|PseudoCAP|PA5556
TIGR NTL03PA05557
UniParc UPI00000C6070
UniProtKB Acc Q9HT18
UniProtKB ID ATPA_PSEAE
UniRef100 UniRef100_A6VF34
UniRef50 UniRef50_P92549
UniRef90 UniRef90_Q87TT2

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP synthase alpha chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (55.39 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.35
Kyte-Doolittle Hydrophobicity Value -0.073
Molecular Weight (kDa) 55.4
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 666 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005087 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5556
Search term: atpA

Human Homologs

Ensembl 94, assembly GRCh38.p12
ATP synthase F1 subunit alpha [Source:HGNC Symbol;Acc:HGNC:823]
E-value: 2.7e-158
Percent Identity: 56.4

References

Nucleotide sequence of the genes coding for alpha, beta and gamma subunits of the proton-translocating ATPase of Escherichia coli.
Kanazawa H, Kayano T, Mabuchi K, Futai M
Biochem. Biophys. Res. Commun. 1981 Nov;103(2):604-12
PubMed ID: 6277310
Directed mutagenesis of the strongly conserved lysine 175 in the proposed nucleotide-binding domain of alpha-subunit from Escherichia coli F1-ATPase.
Rao R, Pagan J, Senior AE
J. Biol. Chem. 1988 Nov;263(31):15957-63
PubMed ID: 2903146
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344