Pseudomonas aeruginosa PAO1, PA5557 (atpH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5557
Name
atpH
Synonym: uncH
Synonym: papE
Replicon chromosome
Genomic location 6252159 - 6252695 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_254244.1
GI 15600750
Affymetrix PA5557_atpH_at
DNASU PaCD00007315
Entrez 878509
GenBank AAG08942.1
INSDC AAG08942.1
NCBI Locus Tag PA5557
protein_id(GenBank) gb|AAG08942.1|AE004967_13|gnl|PseudoCAP|PA5557
TIGR NTL03PA05558
UniParc UPI00000C6071
UniProtKB Acc Q9HT17
UniProtKB ID ATPD_PSEAE
UniRef100 UniRef100_A6VF35
UniRef50 UniRef50_A6VF35
UniRef90 UniRef90_A6VF35

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP synthase delta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (19.26 kDa, PMID:21751344)
Charge (pH 7) -1.55
Kyte-Doolittle Hydrophobicity Value -0.142
Molecular Weight (kDa) 19.3
Isoelectric Point (pI) 5.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Periplasmic Class 1
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 459 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005088 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5557
Search term: atpH

References

The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase.
Gay NJ, Walker JE
Nucleic Acids Res 1981 Aug 25;9(16):3919-26
PubMed ID: 6272190
Solution structure of the N-terminal domain of the delta subunit of the E. coli ATPsynthase.
Wilkens S, Dunn SD, Chandler J, Dahlquist FW, Capaldi RA
Nat. Struct. Biol. 1997 Mar;4(3):198-201
PubMed ID: 9164460
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344