Pseudomonas aeruginosa PAO1, PA0019 (def)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0019
Name
def
Replicon chromosome
Genomic location 21067 - 21573 (- strand)

Cross-References

RefSeq NP_248709.1
GI 15595217
Affymetrix PA0019_def_at
DNASU PaCD00006011
Entrez 879306
GenBank AAG03409.1
INSDC AAG03409.1
NCBI Locus Tag PA0019
protein_id(GenBank) gb|AAG03409.1|AE004441_10|gnl|PseudoCAP|PA0019
TIGR NTL03PA00020
UniParc UPI000012915C
UniProtKB Acc Q9I7A8
UniProtKB ID DEF_PSEAE
UniRef100 UniRef100_Q9I7A8
UniRef50 UniRef50_Q9I7A8
UniRef90 UniRef90_Q9I7A8

Product

Feature Type CDS
Coding Frame 1
Product Name
polypeptide deformylase
Product Name Confidence: Class 2
Synonyms formylmethionine deformylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.13
Kyte-Doolittle Hydrophobicity Value -0.441
Molecular Weight (kDa) 19.4
Isoelectric Point (pI) 4.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1N5N HYDROLASE 11/06/02 Crystal Structure of Peptide Deformylase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.4
1S17 HYDROLASE 01/05/04 Identification of Novel Potent Bicyclic Peptide Deformylase Inhibitors PSEUDOMONAS AERUGINOSA 1.95 X-RAY DIFFRACTION 99.4
1LRY HYDROLASE 05/16/02 Crystal Structure of P. aeruginosa Peptide Deformylase Complexed with Antibiotic Actinonin PSEUDOMONAS AERUGINOSA 2.6 X-RAY DIFFRACTION 100.0
1IX1 HYDROLASE 06/07/02 Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin PSEUDOMONAS AERUGINOSA 1.85 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
2-(3,4-Dihydro-3-Oxo-2h-Benzo[B][1,4]Thiazin-2-Yl)-N-Hydroxyacetamide DB02036 DrugBank 5.0.6 Q9I7A8 Peptide deformylase 100.0 168 7.0e-92
2-[(Formyl-Hydroxy-Amino)-Methyl]-Heptanoic Acid [1-(2-Hydroxymethyl-Pyrrolidine-1-Carbonyl)-2-Methyl-Propyl]-Amide DB04310 DrugBank 5.0.6 Q9I7A8 Peptide deformylase 100.0 168 7.0e-92
N-(2-Acetamido)Iminodiacetic Acid DB02810 DrugBank 5.0.6 Q9I7A8 Peptide deformylase 100.0 168 7.0e-92

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000019 (562 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0019
Search term: def

Human Homologs

Ensembl 86, assembly GRCh38.p7
peptide deformylase (mitochondrial) [Source:HGNC Symbol;Acc:HGNC:30012]
E-value: 6.5e-15
Percent Identity: 32.7

References

A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.
Meinnel T, Blanquet S, Dardel F
J. Mol. Biol. 1996 Sep;262(3):375-86
PubMed ID: 8845003